Cytogenetic Pattern in Adult Patients with de novo Acute Myeloid Leukaemia: a Single Center Study in Bangladesh
*Baqi SA,1 Munmun UK,2 Khan MR,3 Shah MS,4 Islam S,5 Rahman F,6 Aziz MA,7 Begum M8
- Saqi Md. Abdul Baqi, Resident, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka. firstname.lastname@example.org
- Umme Kulsum Munmun, Lecturer, Department of Pathology, Dhaka Medical College, Dhaka.
- Md. Rafiquzzaman Khan, Associate Professor, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
- Md. Salahuddin Shah, Associate Professor, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
- Shafiqul Islam, Medical Officer, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
- Farzana Rahman, Assistant Professor, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
- Md. Abdul Aziz, Professor, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
- Masuda Begum, Professor, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka,
Background: Cytogenetic analysis performed at diagnosis is considered to be the most important prognostic factor in AML.
Objective: The purpose of this study was to observe the pattern of cytogenetic abnormalities in adult patients with de novo AML.
Method: Total fifty two newly diagnosed de novo AML patients were selected for the study. Six cytogenetic abnormalities including t(8;21), t(15;17), inv(16), BCR-ABL1, FLT3-ITD & NPM1 mutations were detected by Real-Time PCR.
Results: In this study, 36 (69.2%) patients showed different cytogenetic abnormalities. The t(15;17) was found to be the most common. t(15;17), t(8;21) and inv(16) were found only in M3, M2 and M4 subtypes, respectively. Significant association was found with increasing age and cytogenetic risk groups. BCR-ABL1 mutation showed significant relation with increased age. FLT3-ITD mutation showed significant association with increased WBC count and inv16 was significantly associated with relatively less bone marrow blast percentage.
Conclusion: So, Cytogenetic study should be performed routinely in all cases of AML for proper diagnosis, prediction of prognosis and implementation of effective therapeutic measures.
[Journal of Histopathology and Cytopathology, 2019 Jul; 3 (2):86-92]
Key words: Acute myeloid leukaemia, cytogenetics
Acute myeloid leukaemia (AML) is a clonal, malignant disease of haematopoietic tissue which is characterized by accumulation of abnormal (leukaemic) blast cells, principally in the bone marrow, and impaired production of normal blood cells.1The incidence of AML is higher in the white population (3.8 per 100,000 person) than that of the Asian population (3.2 per 100,000 person).2 AML is the most common acute leukaemia in adult and it is more common in males. Majority of the AML patients are older than 60 years.3 Cytogenetic abnormalities were first described in AML in the 1960s.4 In 1988, the Morphologic, Immunologic and Cytogenetic (MIC) classification was published. Since then, cytogenetic investigations have become more important in AML classification.5 AML is heterogeneous in terms of morphology, immunophenotype, cytogenetics and molecular genetics.6 Using the WHO criteria, the diagnosis of AML is established by the presence of 20% or more of leukaemic myeloblasts in the peripheral blood or bone marrow. However, in a subgroup of AML, the presence of recurrent genetic abnormalities alone is sufficient for the diagnosis of AML regardless of the blast percentage.7 Geographical, ethnic, and environmental influences should be considered in determining the cytogenetic and morphological features of this disease.8 Cytogenetic abnormalities are identified in 50-60% of adult newly diagnosed AML patients.9 Age and cytogenetic abnormalities are the most important prognostic factors in AML.10There is an increase in the proportion of patients with unfavourable risk cytogenetics and a decrease in favourable risk cytogenetics with advancing age.11 Besides the prognostic importance, cytogenetic analysis is also recommended to monitor minimal residual disease (MRD) in case of AML patients with abnormal cytogenetics.12
From these points of view, the current study was designed to observe the pattern of cytogenetic abnormalities in adult patients with de novo AML, to categorize the patients into three risk groups and to observe the association of cytogenetic findings with FAB subtypes, age, sex & other laboratory findings.
This study was conducted in the department of Haematology, BSMMU from February 2018 to January 2019. Total fifty two newly diagnosed adult patients with de novo AML had been enrolled for the study. Complete blood count was done by automated cell counter machine and checked manually. Morphological diagnosis of AML had been done by bone marrow study. For cytogenetic study, 3-5 ml of peripheral blood or bone marrow aspirate was collected in EDTA tube. Six cytogenetic abnormalities including t(8;21), t(15;17), inv(16), BCR-ABL1, FLT3-ITD & NPM1 mutations were detected on an internationally standard laboratory by Real-Time PCR technique.The ABI 7500 real time PCR system (using the Taqman chemistry) was used for doing the cytogenetic analysis. A pre-designed semi-structured data collection sheet was used for data collection. The statistical analysis was carried out using the SPSS version 24.0 for Windows. For all statistical tests, p-value less than 0.05 was considered as statistically significant.
The mean age of the patients was 36.87 (+13.29) years. Maximum (30.8%) patients belonged to 18-27 years age group. Female was slightly predominant with a male: female ratio was 1: 1.08. Maximum patients belonged to FAB M2 (32.7%), followed by M3 (26.9%) and M1 (17.3%). Among the 52 cases, 36 (69.2%) cases showed different cytogenetic abnormalities and 16 (30.8%) cases had normal cytogenetics. The t(15;17) was found to be the most common detected in 13(25%) patients. NPM1 mutation was found in 10 (19.23%), t(8;21) in 8 (15.38%) and FLT3-ITD mutation in 8 (15.38%) cases (Table I).
M0 and M6 subtypes did not show any cytogenetic abnormality. Most of the M1 cases also had normal cytogenetic pattern. The t(15;17), t(8;21) & inv16 were found only in M3, M2 & M4 subtypes respectively. Only one case showed BCR-ABL1 mutation which belongs to M1 subtype. A combination of NPM1 & FLT3-ITD mutation was found in M4, M1, M3 and M2 subtypes (Table II).
Significant association was found between the mean age of 78 years and BCR-ABL1 mutation (p value 0.038). Favourable cytogenetics were more frequent in younger age groups. Intermediate and unfavourable cytogenetics were common in relatively older age groups. These findings were statistically significant (p value 0.004).
FLT3-ITD mutation was significantly associated with increased WBC count (p value 0.042). inv(16) was significantly associated with relatively less bone marrow blast percentage (p value 0.029). Patients with normal cytogenetics showed significant association with relatively increased bone marrow blast percentage (p value 0.033). No other parameter (sex, haemoglobin level, platelet count) showed any significant association with cytogenetic pattern.
AML is characterized by a spectrum of clinical, morphological, immunophenotypic and associated cytogenetic abnormalities. In this study the mean age at presentation of AML was 36.87 years. Studies in most of the other countries showed much higher mean age.6,8,13,14,15 The reason for this difference may be due to inclusion of only de novo cases of AML in this study or may be geographic/ethnic influence.
Cytogenetic abnormalities were detected in 69.2% of patients with an increased frequency of t(15;17) in this study. These findings are consistent with the results of several other studies conducted by Ayesh et al. (2012)16 and Enjeti et al. (2004).13 Here, favourable cytogenetic risk group belonged to young patients and frequency of unfavourable cytogenetics were higher in older age groups. Similarly, Meng et al. (2013) found 75% favourable cytogenetics in young patients and mostly complex karyotype in elderly patients.6
Male: female ratio in this study was 1:1.08. This finding differs from most of the studies in other countries where male predominance was found.6,13,17This difference is probably due to greater proportion of female bed in our department. Geographic or ethnic influence may also be responsible. An increased frequency of t(15;17) (28%) was found among the male patients in this study. Enjeti et al. (2004) also found increased frequency of t(15;17) among male patients in a study from Singapore.13 No other significant difference was found in this study among male and female patients in relation to cytogenetics.
Cases with FLT3-ITD mutation were found in this study to have increased WBC count. Penget al. (2008) also demonstrated a correlation between the presence of FLT3 mutation and the increased WBC count.18 Similarly, Schnittger et al. (2002) and Haferlach et al. (2012) reported NPM1 and FLT3 mutations to be correlated with higher peripheral WBC count.19,20
The t(15;17) was found to be the most common (25%) cytogenetic abnormality in this study. The frequency of t(15;17) found in this study is much higher than other parts of Asia, like Taiwan (15%), compared to those observed in North America and Europe (3-10%).21,22 The t(15;17) was found in 85.7% of M3 subtype patients in the current study, which is very close to the findings in Singapore (82.5% of M3 patients)13, compared to Japan (75.4%)8 and Europe (72.5%).22
On the other hand, t(8;21) was found in 41.2% of AML M2 patients in this study. This percentage is closer to the incidence found in Japanese and Taiwanese reports (33.1% and 34% of their AML M2 patients).8,21 The t(8;21) is less frequent among the AML M2 patients in Singapore (14.5%), Australia (15.3%) and North America (22%).13,23,24
In this study, significant difference was found in case of inv(16) pattern between M4 and non-M4 subtypes. Enjeti et al. (2004) also found the highest frequency of inv16 among their M4 cases.13
FLT3-ITD mutation was found more frequently in M4 subtype in this study. Similarly, another study carried out by Koczkodaj et al. (2016) in Southeastern Poland found the highest frequency of FLT3-ITD mutation in AML M4 cases.25 In an Iranian study, Rezaei et al (2017) divided the patients into FAB M3 and non-M3 groups and analyzed the FLT3-ITD and NPM1 mutational status among these patients. They also found that these mutations were more frequent in non-M3 patients.26 On the other hand, Smith et al. (2011) found a higher frequency of FLT3-ITD mutations among M3 patients.27
Falini et al. (2005) observed that FLT3-ITD mutations occur twice as often in the cases with simultaneous NPM1 mutations as in the cases without this mutation.28 The current study also shows that co-existent FLT3-ITD & NPM1 mutations are more frequent than FLT3-ITD mutation alone.
These differences in the frequency of cytogenetic/molecular genetic abnormalities might be due to geographic & ethnic heterogenecity, variation in the sample size or inclusion of paediatric patients in some studies. Paediatric patients were excluded in our study.
Significant number of patients showed different cytogenetic abnormalities. Therefore, cytogenetic study should be performed routinely in all cases of AML for proper diagnosis, prediction of prognosis and implementation of effective therapeutic measures.
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