Author: bapath

Posted on

jhc2025v9i1s8

Original Article

Histopathological Study of Clinically Suspected Marjolin Ulcer at a Specialized Major Burn Centre in Bangladesh

 

*Asaduzzaman,1 Datta T,2 Khandkar T,3  Chowdhury MA4

 

 

  1. *Dr. Asaduzzaman, Associate Professor of Pathology, National Institute of Burn and Plastic Surgery, Dhaka. dr.asad37@gmail.com
  2. Tanusree Datta, Junior Consultant (Current Charge), National Institute of Burn and Plastic Surgery, Dhaka
  3. Tahmina Khandkar, Junior Consultant, Paediatrics, National Institute of Burn and Plastic Surgery, Dhaka
  4. Mehdi Ashik Chowdhury, Associate Professor of Pathology, Tairunnessa Memorial Medical College.

 

*For correspondence

Abstract

Background: Marjolin ulcer is a malignant transformation arising from chronic ulcers or scars from previously traumatized tissues that occur usually after burns. This study aims to study the histopathological profile of a suspected Marjolin ulcerat a major burn center in Bangladesh.

Methods: A retrospective analysis of all suspected Marjolin ulcer patients presented at theNational Institute of Burn and Plastic Surgery, Dhaka, Bangladesh from 2020 to 2024 was done. A total of 92 patients of all age groups were included in the study.

Results: Most of the patients were between 31-60 years with an overall male preponderance. The most common cause for Marjolin ulcer was burn scars followed by trauma. Lower extremities were found to be the most predominant site. The latency period for the development of Marjolin ulcer was more than 20 years in most of the cases. Squamous cell carcinoma was the most common histological subtype.

Conclusion: Chronic non-healing ulcers or scars from previous trauma that do not respond to treatment should be carefully examined for malignant transformation. Histopathologically, these Marjolin ulcers in most of cases are squamous cell carcinoma.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):55-63]

DOI: https://www.doi.org/10.69950/jhc2025v9i1s8

 

Keywords: Burn; Marjolin Ulcer; Basal cell carcinoma; Squamous Cell Carcinoma

Posted on

jhc2025v9i1s7

Original

Computed Tomography Guided Fine Needle Aspiration Cytology of Mass Lesions of Lung: A Study of 56 Cases

*Wahed A,1 Islam N,2 Hossain MM,3 Nurunnabi M4

 

  1. *Dr. Abdul Wahed, Lecturer, Department of Pathology, Tangail Medical College, Tangail 1900, Bangladesh. shouravsomc88@gmail.com, ORCID: https://orcid.org/0009-0004-6273-0145
  2. Nazmul Islam, Associate Professor, Department of Pathology, Army Medical College Cumilla, Cumilla 3500, Bangladesh. nazmul47@gmail.com
  3. Mohammad Mosharaf Hossain, Assistant Professor, Department of Medicine, Tangail Medical College, Tangail 1900, Bangladesh. mosharaf51@gmail.com
  4. Mohammad Nurunnabi, Assistant Professor, Department of Community Medicine and Public Health, Sylhet Women’s Medical College, Sylhet 3100, Bangladesh. nur.somch@gmail.com

 

*For correspondence

Abstract

Background: Fine needle aspiration cytology (FNAC) determines the type of tumour and affirms the diagnosis. Computed tomography (CT)-guided FNAC of suspicious lung masses is a widely employed, inexpensive diagnostic procedure.

Objective: To evaluate the age, sex, topographic distribution, size, and cytopathological diagnosis of lung mass lesions using CT-guided FNAC.

Methods: This descriptive cross-sectional study included 56 individuals with pulmonary mass lesions, most of which were presumably caused by neoplastic disease, as evidenced by chest radiographs and CT scans. The study was conducted between June 2022 and June 2024.

Results: The mean age of the study participants was 63.6±11.8 years, with more than half (51.8%) falling within the 46–65 age group. Lesions were more commonly found in the right lung (57.1%) compared to the left lung (42.9%). The upper lobe accounted for about two-thirds of the lesions (64.3%), while the middle lobe had the fewest (3.6%). Of all cases, 87.5% were malignant, and 12.5% were benign. The most common malignant lesions were squamous cell carcinoma (33.9%), adenocarcinoma (28.6%), and poorly differentiated carcinomas (21.4%). Small cell carcinoma was identified in 3.6% of cases. There was a statistically significant association between age group and type of malignancy (P<0.05), with the 46–65 age group showing a higher prevalence of malignant lesions (55.1%).

Conclusion: CT-guided FNAC is a highly effective and comparatively accurate diagnostic technique for pulmonary mass lesions, with a permissible complication risk.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):48-54]

DOI: https://www.doi.org/10.69950/jhc2025v9i1s7

 

Keywords: FNAC, CT-guided FNAC, pulmonary mass lesions, Tangail, Bangladesh.

 

Posted on

jhc2025v9i1s6

Original article

Expression of Ki-67 and AgNOR in Primary Central Nervous System (CNS) Tumours

*Nahar A,1 Islam MN,2 Kabir E3

 

  1. *Dr. Asrafun Nahar, MBBS, MD(Pathology), Assistant Professor, Department of Pathology, National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207. dr.asrafunnahar@gmail.com.
  2. Md. Nasimul Islam, MBBS, M.Phil (Pathology), Professor, Department of Pathology, Anwer Khan Modern Medical College, Dhanmondi, Dhaka.
  3. Enamul Kabir, MBBS, M. Phil, M. Sc(Pathology), Professor and Head, Department of Pathology, Popular Medical College, Dhanmondi, Dhaka

 

* For correspondence

Abstract

Background: Primary tumors in central Nervous System (CNS) include a large group of tumors. Ki-67 is usually used for determining proliferative activity, though it has some limitations. It is proved that the number or size of Argyrophilic Nucleolar Organizer Regions (AgNORs) of DNA closely correlates with cellular proliferating activities.

Objective: This study was conducted to find the correlation of expression of Ki-67 and AgNOR with different grades of primary CNS tumours.

Method: In this cross sectional study a total 85 cases with wide age range (2-70 years) were included based on selection criteria. Tissue fixation, processing, staining (hematoxylin and eosin stain, and AgNOR special stain) and immunohistochemistry were done according to the standard protocol followed by the pathology department of Sir Salimullah Medical College (SSMC) and National Institute of Neurosciences & Hospital (NINS), Dhaka.

Results: Ki-67 labeling index and mean AgNOR (mAgNOR) showed significant correlation with different grades of primary CNS tumours, as the grades increased both parameters were also increased. In intergrade comparisons, Ki-67 labeling index showed significant correlation among all the grades except between grade I & grade II and grade III & grade IV. Whereas, mean AgNOR showed significant difference among all the grades including the difference between grade I & II and grade III & IV. Positive significant correlation seen in between ki-67 Labeling Index (LI) and mean AgNOR, where the value of Spearman’s correlation coefficient was 0.514 and p value was 0.001

Conclusion: Both Ki-67 expression and mAgNOR score were increased with the increasing grade of tumours from low to high grade and positive correlation between these two parameters were also found.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):38-47]

DOI: https://www.doi.org/10.69950/jhc2025v9i1s6

 

Keywords: Primary CNS tumours, mAgNOR, Ki-67 labeling index