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JHC 2023 July v7 i2 s4

Original Contribution

Evaluation of Cyclin D1 Expression in Paediatric Common Solid Small
Round Cell Tumours

1.Dr. Md. Shahrior Nahid, MBBS, MD (Pathology), Medical Officer, Department of Pathology, National
Institute of Laboratory Medicine and Referral Centre (NILMRC), Dhaka-1207. shahrior.nahid@gmail.com
2. Prof. (Dr.) Ferdousy Begum, MBBS, MD (Pathology), Professor, Department of Pathology, Bangabandhu
Sheikh Mujib Medical University, Dhaka-1000.
3. Prof. (Dr.) Mohammed Shahed Ali Jinnah, MBBS, MD(Pathology), Professor, Department of Pathology,
National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207.
4. Dr. Saumitra Chakravarty, MBBS, MD(Pathology), Assistant Professor, Department of Pathology,
Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
5. Dr. Arbin Siddiquea, MBBS, MD(Biochemistry), Lecturer, Department of Biochemistry, Shaheed
Suhrawardy Medical College, Dhaka-1207.
6. Dr. Abu Saeed Bin Hasem, MBBS, MD (Pathology), Lecturer, Department of Pathology, Sylhet MAG
Osmani Medical College.
7. Dr. Md. Shajjad Hossain, MBBS, MD(Pathology), Lecturer, Department of Pathology, Dhaka Dental
College, Dhaka.⃰
For correspondence

Abstract
Background: Small round blue cell tumors (SRBCTs) in children are a heterogeneous group of
neoplasms. Their diagnosis is very difficult due to their primitive character. Although the advent
of immunohistochemistry has improved the quality of diagnosis, some cases require molecular
analysis. However, the application of molecular tests is limited due to the lack of resources.
Nuclear expression of Cyclin D1 can be used as a diagnostic adjunct to conventional markers in
diagnosing small round cell tumors, especially when the diagnosis becomes difficult even
following the application of conventional markers.
Objectives: This study aimed to evaluate the immunoreactivity of Cyclin D1 in the common solid
small round blue cell tumors found in children below 15 years of age.
Methods: In this descriptive cross-sectional study, 64 confirmed (immunohistochemically and/or
morphologically) cases of SRBCTs including, Ewing sarcoma (31.25%), neuroblastoma (23.44%),
lymphoblastic lymphoma (21.87%), rhabdomyosarcoma (15.62%) and Wilms tumor (7.81%) in
children under 15 years were selected as samples by inclusion and exclusion criteria. Finally, the
immunoreactivity of Cyclin D1 in each case was assessed on the basis of staining pattern and
staining intensity.
Result: All cases of Ewing sarcoma and neuroblastoma exhibited nuclear expression for cyclin
D1. Seventy percent (14/20) of cases of Ewing sarcoma and 66.67% (10/15) of neuroblastoma
cases showed diffuse nuclear expression. Seventy percent (14/20) of cases of Ewing sarcoma and
93.33% (14/15) of neuroblastoma cases showed strong staining intensity (3+). In contrast, this
marker showed a negative reaction in rhabdomyosarcoma and lymphoblastic lymphoma, while it
was focally positive with moderate intensity in the blastemal component of 40% of cases of Wilms
tumor.
Conclusion: The present study suggests that cyclin D1 can be exploitable as a diagnostic adjunct
to conventional markers in confirming the diagnosis of Ewing sarcoma or Neuroblastoma.

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):69-79]
Keywords: Small round blue cell tumors (SRBCTs), Cyclin D1

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JHC 2023 July v7 i2 s3

Original Contribution

Histomorphological Patterns of Different Breast Lesions in a Tertiary Care Hospital

1. *Dr. Tanshina Afrin, MD (Pathology), Assistant Professor (C.C), Pathology Department, Rajshahi Medical
College. tanshinaafrin@gmail.com
2. Prof. Dr. S M Asafudullah, Professor and Head of Department of Pathology, Rajshahi Medical College
3. Dr. Khadiza Khanam, Professor, Pathology Department, Rajshahi Medical College.
4. Dr. Arefa Sultana, Associate Professor, Pathology Department, Rajshahi Medical College.
5. Dr. Anindita Sarkar, Assistant Professor (C.C), Pathology Department, Rajshahi Medical College.
6. Dr. Nazifa Islam, Assistant Professor (C.C), Pathology Department, Rajshahi Medical College.
7. Dr. Samrose Sultana, Assistant Professor (C.C) Pathology Department, Rajshahi Medical College.
* For correspondence

Abstract
Objective: Breast lesions are heterogeneous diseases that consist of several distinct entities
with remarkably different characteristic features. The present study was conducted to assess
histopathological findings of breast lesions in excision biopsy samples.
Methods: This cross-sectional study was conducted in the Department of Pathology, Rajshahi
Medical College over a period of one year from July 2021 to June 2022. A total of 105 cases of
breast lesions were included in this study.
Result: Out of 105 cases of breast lesions studied, 59 cases (72%) were malignant and 46 cases
were benign lesions. Fibroadenoma (18 cases out of 46) was the most common benign lesion
followed by breast abscess (9 cases out of 46). On the other hand, invasive ductal carcinoma
was the highest occurred malignant tumour (59 cases, 56%). The age range of breast lesion was
15 to 75 yrs and highest incidence was in between 31-50 years (both benign and malignant).
Most of the breast lesions were occurred in left breast (62%).
Conclusion: Histopathological examination plays an important role in differentiating between
benign and malignant lesions. In the present study the most common benign breast lesion was
fibroadenoma and the most common malignant lesion was infiltrating ductal carcinoma. The

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):62-68]
Keywords: Histopathology, Malignant, Benign lesions, Breast lesion

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JHC 2023 v7 i2 s2

Original Contribution

Ultrasound Guided Fine Needle Aspiration Cytology of Lung
Lesions

1. *Dr. Rukhshana Akhter Jahan, Associate Professor, Department of Pathology, Rajshahi Medical College,
Rajshahi. jahanrukhshana@gmail.com
2. Professor Dr. S M Asafudullah, Professor & Head, Department of Pathology, Rajshahi Medical College,
Rajshahi. drshamol@gmail.com
3. Dr. Rebeka Sultana, Consultant Pathologist, Raj Pathology, Rajshahi. doctorrebekasultana@gmail.com
4. Dr. S. M. Moshaddeq Hossain, Assistant Professor, Department of Pathology, Rajshahi Medical College,
Rajshahi. mridulrmcbd@yahoo.com
5. Dr. Samrose Sultana, Assistant Professor, Department of Pathology, Pabna Medical College, Pabna.
samrosesina@gmail.com

Abstract
Background: This study was aimed to evaluate the role of transthoracic ultrasound guided
FNAC in diagnosis of peripheral lung lesion. It is an important and useful investigation to
differentiate between benign and malignant lesions of lung. USG guided FNAC of lung lesion
is a safe, radiation free, and cost effective method. Besides, the procedure is simple and
complications if occur, can be managed conservatively.
Methods: This was a cross-sectional study conducted at the department of Pathology,
Rajshahi Medical College. Some samples were also collected from a private diagnostic centre,
Rajshahi over a period of one year from January 2021 to December 2021. 150 patients who
fulfilled inclusion criteria were included in this study. After properly explaining the procedure
and taking informed consent, USG guided FNAC was done in all the patients. Radiological
and cytological data of enrolled patients was collected prospectively and analyzed.
Results: Among the 150 patients, this study comprising of 120 male and 30 female in age
range of 21 to 90 years. Malignancy was the most common cytological diagnosis (76%) while
as benign diagnosis was reached in 22.66%. In 1.33% of patients, FNAC was inconclusive.
Squamous cell carcinoma was the most common malignancy diagnosed in 46(30.66%)
patients, followed by adonocarcinoma 31(20.66%), 19(12.66%) patients had undifferentiated
carcinoma, metastatic carcinoma 09(06%), small cell carcinoma 07(4.66%), and Non Hodgkin
lymphoma was diagnosed in 02(1.33%) patients. Among the benign group, nonspecific
chronic inflammation was diagnosed in 18(18%) patients, tuberculous granuloma 07(4.66%),
while the smear was inconclusive in 02(1.33%) patients.
Conclusion: Ultrasound guided fine needle aspiration cytology is safe, less expensive, less
time consuming, less invasive diagnostic tool with high degree of accuracy and no radiation
toxicity to lung lesions

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):55-61]
Keywords: Malignancy, Tuberculosis, USG guided Fine needle aspiration cytology.

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