Tag: Cyclin D1

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JHC 2023 July v7 i2 s4

Original Contribution

Evaluation of Cyclin D1 Expression in Paediatric Common Solid Small
Round Cell Tumours

1.Dr. Md. Shahrior Nahid, MBBS, MD (Pathology), Medical Officer, Department of Pathology, National
Institute of Laboratory Medicine and Referral Centre (NILMRC), Dhaka-1207. shahrior.nahid@gmail.com
2. Prof. (Dr.) Ferdousy Begum, MBBS, MD (Pathology), Professor, Department of Pathology, Bangabandhu
Sheikh Mujib Medical University, Dhaka-1000.
3. Prof. (Dr.) Mohammed Shahed Ali Jinnah, MBBS, MD(Pathology), Professor, Department of Pathology,
National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207.
4. Dr. Saumitra Chakravarty, MBBS, MD(Pathology), Assistant Professor, Department of Pathology,
Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
5. Dr. Arbin Siddiquea, MBBS, MD(Biochemistry), Lecturer, Department of Biochemistry, Shaheed
Suhrawardy Medical College, Dhaka-1207.
6. Dr. Abu Saeed Bin Hasem, MBBS, MD (Pathology), Lecturer, Department of Pathology, Sylhet MAG
Osmani Medical College.
7. Dr. Md. Shajjad Hossain, MBBS, MD(Pathology), Lecturer, Department of Pathology, Dhaka Dental
College, Dhaka.⃰
For correspondence

Abstract
Background: Small round blue cell tumors (SRBCTs) in children are a heterogeneous group of
neoplasms. Their diagnosis is very difficult due to their primitive character. Although the advent
of immunohistochemistry has improved the quality of diagnosis, some cases require molecular
analysis. However, the application of molecular tests is limited due to the lack of resources.
Nuclear expression of Cyclin D1 can be used as a diagnostic adjunct to conventional markers in
diagnosing small round cell tumors, especially when the diagnosis becomes difficult even
following the application of conventional markers.
Objectives: This study aimed to evaluate the immunoreactivity of Cyclin D1 in the common solid
small round blue cell tumors found in children below 15 years of age.
Methods: In this descriptive cross-sectional study, 64 confirmed (immunohistochemically and/or
morphologically) cases of SRBCTs including, Ewing sarcoma (31.25%), neuroblastoma (23.44%),
lymphoblastic lymphoma (21.87%), rhabdomyosarcoma (15.62%) and Wilms tumor (7.81%) in
children under 15 years were selected as samples by inclusion and exclusion criteria. Finally, the
immunoreactivity of Cyclin D1 in each case was assessed on the basis of staining pattern and
staining intensity.
Result: All cases of Ewing sarcoma and neuroblastoma exhibited nuclear expression for cyclin
D1. Seventy percent (14/20) of cases of Ewing sarcoma and 66.67% (10/15) of neuroblastoma
cases showed diffuse nuclear expression. Seventy percent (14/20) of cases of Ewing sarcoma and
93.33% (14/15) of neuroblastoma cases showed strong staining intensity (3+). In contrast, this
marker showed a negative reaction in rhabdomyosarcoma and lymphoblastic lymphoma, while it
was focally positive with moderate intensity in the blastemal component of 40% of cases of Wilms
tumor.
Conclusion: The present study suggests that cyclin D1 can be exploitable as a diagnostic adjunct
to conventional markers in confirming the diagnosis of Ewing sarcoma or Neuroblastoma.

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):69-79]
Keywords: Small round blue cell tumors (SRBCTs), Cyclin D1

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jhc.2024.8.1.07

Journal of Histopathology and Cytopathology

January 2024 Volume 8 Issue 1

Original Contribution

Cyclin D1 Expression in Different Grades of Oral Squamous Cell Carcinoma: A Study Based on Small Biopsy Samples

1. *Dr. Abu Saeed Bin Hasem, MBBS, MD (Pathology), Lecturer, Department of Pathology, Sylhet MAG Osmani Medical College. saeedbinhasem@gmail.com.
2. Professor (Dr.) Azizul Qadar Md. Abdul Hye, M. Phil (Pathology), Former-Head and Professor, Department of Pathology, Sylhet MAG Osmani Medical College.
3. Professor (Dr.) Shamim Akhter Mimi, M. Phil (Pathology), Head and Professor, Department of Pathology, Sylhet MAG Osmani Medical College.
4. Dr. Md. Mizanur Rahman, MBBS, MD (Pathology), Assistant Professor, Pabna Medical College, Pabna.
5. Dr. Md. Shahrior Nahid, MBBS, MD (Pathology), Resident Medical Officer, National Institute of Laboratory Medicine and Referral Centre, Sher-e-Bangla Nagar, Agargaon, Dhaka-1207.
6. Dr. Umama-Tun-Nesa Emita, MBBS, MD (Pathology), Pathologist, Khulna Medical College Hospital, Khulna.
7. Dr. Sadia Afroz, MBBS, MD (Pathology), Medical Officer, Department of Histopathology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka-1212.

* For correspondence

Abstract
Background: Cyclin D1 oncogene plays a critical role in various malignancies by regulating the G1/S transition of the cell cycle. Overexpression of cyclin D1 in oral squamous cell carcinoma is associated with poor prognosis and resistance to cisplatin-based conventional chemotherapy.

Objectives:To assess the immunohistochemical expression of cyclin D1 in different grades of oral squamous cell carcinoma (OSCC) and to determine the association of cyclin D1 expression with histopathological grades, age, gender, anatomic site, and the habit of using tobacco, betel quid and areca nut of OSCC patients.
Methods: This cross-sectional observational study was conducted at the Department of Pathology, Sylhet MAG Osmani Medical College, Sylhet during the study period (from March, 2019 to April, 2020 and July, 2021 to April, 2022). A total of 52 small biopsy cases were processed; paraffin blocks were made and stained with routine H&E stain. The sections were examined microscopically and the tumors were graded histologically. Immunohistochemistry was performed by using a commercially available anti-cyclin D1 antibody. Cyclin D1 overexpression was considered when more than 10% of tumor cells displayed nuclear staining with moderate to strong intensity.
Results: Cyclin D1 overexpression was seen in 60% of cases of OSCC. The highest expression was seen in moderately differentiated followed by poorly differentiated and well-differentiated squamous cell carcinoma, with no statistically significant correlation. It did not correlate with age, gender, anatomic site, the habit of using tobacco, betel quid, and areca nut chewing.
Conclusion: In Bangladesh, 60% of cases of OSCC showed cyclin D1 overexpression which was quite similar to the findings of other countries. Further, alarge cohort study on resected samples can be done for overall survival and future targeted therapy against cyclin D1.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):49-55]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.07
Keywords: Cyclin D1, Expression, OSCC, Overexpression

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