Tag: Expression

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jhc2025v9i1s2

Original Article

Association of E-cadherin Expression with Histopathological Grades and Clinical Stages of Laryngeal Squamous Cell Carcinoma

*Tayeb M,1Mimi SA,2 Kamal MS,3 Snigdha SS4

  1. *Dr. Mohammad Tayeb, MD (Pathology), Assistant Professor, Department of Pathology, Jahurul Islam Medical College. tmohammad75@yahoo.com
  2. Professor Dr. Shamim Akhter Mimi, M. Phil (Pathology), Professor and Head, Department of Pathology, Sylhet MAG Osmani Medical College.
  3. Mohammad Shah Kamal, FCPS (ENT), Associate Professor, Department of Otorhinology & Head-Neck Surgery, Sylhet MAG Osmani Medical College.
  4. Shyla Sharmin Snigdha, MD (Pathology),Assistant professor, Department of Pathology, Zainul Haque Sikder Women’s Medical College.

*For correspondence

Abstract
Background: E-cadherin gene plays an important role in the carcinogenesis of laryngeal squamous cell carcinoma (LSCC). This study aimed to evaluate the immunohistochemical expression of E-cadherin in different grades and clinical stages of LSCC and to determine the association of E-cadherin with clinical stages and histopathological grades.
Methods: This cross-sectional study was conducted in the Department of Pathology, Sylhet MAG Osmani Medical College, from March 2021 to January 2023. Immunohistochemistry was performed in 50 histopathologically diagnosed cases of LSCC using a commercially available anti-E-cadherin antibody. The total score of immunoreaction was calculated by multiplying the expression score and intensity score.
Results: The mean age of the LSCC patients was 62.8 years, and 66% were male. Grade I is the most frequent histopathological grade (48%), followed by grade II (42%) and grade III (10%). The most frequent clinical T stage was T3 (56%), and the N stage was N2 (58.06%). E-cadherin expression was positive in 72% of cases; the rest, 28%, showed reduced expression. A significant association was found between E-cadherin expression with histopathological grades (p=0.007) and clinical N stage (p=0.009) but not significant with clinical T stage (p=0.502), anatomic site (P=0.132), and the habit of smoking (0.276).

Conclusion: LSCC patients with reduced E-cadherin expression are at the risk of high-grade carcinoma and nodal metastasis. So, the expression of E-cadherin in pre-treatment biopsy samples can be utilized as one of the prognostic factors and advocated to anti-E-cadherin targeted therapy in LSCC.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):3-9]

Keywords: E-cadherin, Expression, Immunohistochemistry, laryngeal squamous cell carcinoma.

DOI: https://www.doi.org/10.69950/jhc2025v9i1s2

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jhc.2024.8.1.07

Journal of Histopathology and Cytopathology

January 2024 Volume 8 Issue 1

Original Contribution

Cyclin D1 Expression in Different Grades of Oral Squamous Cell Carcinoma: A Study Based on Small Biopsy Samples

1. *Dr. Abu Saeed Bin Hasem, MBBS, MD (Pathology), Lecturer, Department of Pathology, Sylhet MAG Osmani Medical College. saeedbinhasem@gmail.com.
2. Professor (Dr.) Azizul Qadar Md. Abdul Hye, M. Phil (Pathology), Former-Head and Professor, Department of Pathology, Sylhet MAG Osmani Medical College.
3. Professor (Dr.) Shamim Akhter Mimi, M. Phil (Pathology), Head and Professor, Department of Pathology, Sylhet MAG Osmani Medical College.
4. Dr. Md. Mizanur Rahman, MBBS, MD (Pathology), Assistant Professor, Pabna Medical College, Pabna.
5. Dr. Md. Shahrior Nahid, MBBS, MD (Pathology), Resident Medical Officer, National Institute of Laboratory Medicine and Referral Centre, Sher-e-Bangla Nagar, Agargaon, Dhaka-1207.
6. Dr. Umama-Tun-Nesa Emita, MBBS, MD (Pathology), Pathologist, Khulna Medical College Hospital, Khulna.
7. Dr. Sadia Afroz, MBBS, MD (Pathology), Medical Officer, Department of Histopathology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka-1212.

* For correspondence

Abstract
Background: Cyclin D1 oncogene plays a critical role in various malignancies by regulating the G1/S transition of the cell cycle. Overexpression of cyclin D1 in oral squamous cell carcinoma is associated with poor prognosis and resistance to cisplatin-based conventional chemotherapy.

Objectives:To assess the immunohistochemical expression of cyclin D1 in different grades of oral squamous cell carcinoma (OSCC) and to determine the association of cyclin D1 expression with histopathological grades, age, gender, anatomic site, and the habit of using tobacco, betel quid and areca nut of OSCC patients.
Methods: This cross-sectional observational study was conducted at the Department of Pathology, Sylhet MAG Osmani Medical College, Sylhet during the study period (from March, 2019 to April, 2020 and July, 2021 to April, 2022). A total of 52 small biopsy cases were processed; paraffin blocks were made and stained with routine H&E stain. The sections were examined microscopically and the tumors were graded histologically. Immunohistochemistry was performed by using a commercially available anti-cyclin D1 antibody. Cyclin D1 overexpression was considered when more than 10% of tumor cells displayed nuclear staining with moderate to strong intensity.
Results: Cyclin D1 overexpression was seen in 60% of cases of OSCC. The highest expression was seen in moderately differentiated followed by poorly differentiated and well-differentiated squamous cell carcinoma, with no statistically significant correlation. It did not correlate with age, gender, anatomic site, the habit of using tobacco, betel quid, and areca nut chewing.
Conclusion: In Bangladesh, 60% of cases of OSCC showed cyclin D1 overexpression which was quite similar to the findings of other countries. Further, alarge cohort study on resected samples can be done for overall survival and future targeted therapy against cyclin D1.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):49-55]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.07
Keywords: Cyclin D1, Expression, OSCC, Overexpression

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