Tag: p53

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jhc.2026.10.1.7

Journal of Histopathology and Cytopathology

Official Journal of Bangladesh Academy of Pathology

Vol 10, No 1, January 2026

Expression of p53 in Urothelial Carcinoma of the Urinary Bladder: Correlation with Tumor Grade and Muscle Invasion
Azim T,1 Jinnah MS,2 Aktar M,3 Ahmed M,4 *Badhan RE5

Abstract
Background: The p53 gene, a well-known tumor suppressor gene located on chromosome 17p13.1, plays a pivotal role in regulating cell cycle arrest and apoptosis. Mutations or overexpression of p53 have been linked to tumor progression in urothelial carcinoma.
Aim: To evaluate p53 expression in urothelial carcinoma of the urinary bladder and determine its relationship with histopathological grade and muscle invasion.
Methods: This cross-sectional study was conducted at Dhaka Medical College between March 2020 and February 2022. Forty histopathologically diagnosed urothelial carcinoma cases from TURBT or cystectomy specimens were included. p53 expression was assessed by immunohistochemistry and correlated with tumor grade and the presence of muscle invasion.
Results: The mean age was 60.85 ± 11.18 years (range 40–85), with a male-to-female ratio of 3.4:1. p53 was positive in 65% of all cases and in 92.3% of high-grade tumors, while most low-grade tumors were negative. Among high-grade, muscle-invasive cases, p53 positivity was observed in 93.3% of cases. The correlation between p53 overexpression and both tumor grade and muscle invasion was statistically significant (p<0.05).
Conclusion: p53 expression is significantly associated with aggressive urothelial carcinoma phenotypes, supporting its utility as a prognostic biomarker in clinical decision-making.
[Journal of Histopathology and Cytopathology, 2026 Jan; 10 (1):53-62]
DOI: https://www.doi.org/10.69950/jhc.2026.10.1.7

Keywords: p53, tumor suppressor gene, bladder cancer, urothelial carcinoma, cell cycle regulation, immunohistochemistry, muscle-invasive

  1. Dr. Tasmia Azim, Assistant Professor, Department of Pathology, Holy Family Red Crescent Medical College, Dhaka. tasmia.azim@gmail.com  
  2. Professor Dr. Mohammed Shahed Ali Jinnah. Ex-director, National Institute of Laboratory Medicine and Referral Centre (NILMRC), Dhaka. jinnah63@gmail.com
  3. Dr. Marufa Aktar, Assistant Professor, Department of Pathology, Sher E Bangla Medical College, Barishal. marufaepu@gmail.com
  4. Dr. Nasim Ahmed, Junior Consultant,Department of Anesthesia, Analgesia, Palliative and Intensive Care Medicine, Dhaka Medical College and Hospital. : dr.ahmednasim@gmail.com
  5. *Dr. Raisa Enayet Badhan, Medical Officer, Department of Microbiology, National Institute of Burn and Plastic Surgery, Dhaka. raisabadhan@gmail.com

*For Correspondence

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jhc2025v9i1s4

Original Article

Expression of P53 in Clinically Diagnosed Solitary Thyroid Nodules

 *Ripa SP,1 Shilpi HK,2 Begum S3

 

  1. *Dr. Sabera Pervin Ripa, Assistant Professor, Department of Pathology, United Medical College, Shatarkul, Dhaka. dr.saberapervin@gmail.com.
  2. Harunur Rashid Khan Shilpi, Professor and ex-Head, Department of Pathology, Sir Salimullah Medical College, Dhaka.
  3. Shahnaj Begum, Professor and Head, Department of Pathology, Sir Salimullah Medical College, Dhaka.

 

*For correspondence

Abstract

Background: Solitary thyroid nodule has provoked increased concern owing to higher incidence of malignancy in it compared to multinodular goiter.Expression of p53 in solitary thyroid nodules has a significant impact on early diagnosis and better treatment strategy of thyroid carcinoma.

Method: Total 40 cases of solitary thyroid nodules were evaluated by immunohistochemical staining for mutant p53 expression. The study was performed in Sir Salimullah Medical College, Dhaka (from January, 2019 to December, 2020). Statistical analyses were carried out by using SPSS version 22 for Windows. A descriptive analysis was performed for all data.

Results: In this study, the mean age was 33.38 ± 13.11 and male to female ratio was 1:9. p53 expression was observed to be found more in relatively smaller tumor and most of the  differentiated tumors. Among malignant lesions, the predominant histologic type was papillary thyroid carcinoma (85%) including follicular variant and usual type. All carcinomas (100%) were well differentiated. Most of malignant lesions (92.6%) showed positive p53 expression.

Conclusion: Patients with p53 expression are related to malignant thyroid nodules. So, p53 immunoexpression is an important genetic marker for differentiating benign and malignant thyroid nodules.

 

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):20-29]

DOI: https://www.doi.org/10.69950/jhc2025v9i1s4

 

Keywords: Solitary thyroid nodule, p53

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jhc.2024.8.1.03

Journal of Histopathology and Cytopathology

January 2024 Volume 8 Issue 1

Original Contribution

Evaluation of Immunohistochemical Expression of p53 in Colorectal Carcinoma

1. *Dr. Mahfuza Jebun  Mouri; Lecturer, Department of Pathology, Shaheed Suhrawardy Medical College, Sher-E-Bangla Nagar, Dhaka.  mouri@gmail.com.
2. Professor Dr. Enamul Kabir, Professor, Department of Pathology, Popular Medical College, Dhaka.
3. Professor Dr. Shahnaj Begum, Professor, Department of Pathology, Sir Salimullah Medical College, Dhaka.

*For correspondence
Abstract
Background: The most common gastrointestinal malignancy is colorectal carcinoma and is a major cause of morbidity and mortality. In colorectal carcinoma the most frequently mutated gene is p53 tumor suppressor gene. Mutation of p53 gene gives rise to abnormal protein which can be easily detected by immunohistochemistry. Expression of mutant p53 protein has been associated with poor clinical outcome and increased risk of death due to increased aggressiveness of the disease.
Objective: The aim of the study was to see the clinicopathological correlation of mutant p53 expression in colorectal carcinoma.
Method: Total 50 paraffin embedded tissue blocks of histopathologically diagnosed cases of colorectal cancer were evaluated by immunohistochemical staining for mutant p53 expression. The study was performed in Sir Salimullah Medical College, Dhaka (from March, 2018 to February, 2020).
Results: Out of 50 patients studied, 29 cases (58%) expressed mutant p53 protein in the nucleus of malignant cells. There was significant association between  p53  protein expression and clinicopathologic variables such as age (<40 years vs >40 years, p=0.032), site of tumor (left vs right colon, p=0.028), pathological type (mucinous vs non mucinous, p=0.039), grade (a greater tendency towards poor differentiation, p= 0.039), advanced stage (both TNM and Dukes), whereas no significant association was found between mutant p53 protien expression and other parameters like gender and morphological types.
Conclusion: The results of this current study revealed that mutant p53 positive colorectal cancer tended to be related to a higher grade of malignancy, advanced tumor stage and mucinous morphology. The results of this current study revealed that mutant p53 positive colorectal cancer tended to be related to a higher grade of malignancy, advanced tumor stage and mucinous morphology. So, p53 is an important immunohistochemical marker for colorectal cancer patients.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):10-18]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.03
Keywords: Colorectal cancer, p53, Immunohistochemistry
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