Original Contribution
Morphological Patterns of Glomerular Diseases among Children Studied by
Light and Immunofluorescence Microscopy at Selected Tertiary Care Hospitals
1.*Dr. Muhammad Ariful Haque, Assistant Professor, Faculty of Laboratory Medicine, ICMH, Matuail, Dhaka.
ariful31st@gmail.com
2. Dr. Habiba Akter, Assistant Professor, Department of Physiology, Khwaja Yunus Ali Medical College, Sirajganj.
3. Dr. SM Basitur Roshid, Assistant Professor, Department of Pathology, BIRDEM, Dhaka.
4. Dr. Md. Shariful Haque, Deputy Program Manager-4, Hospital Services Management, DGHS, Dhaka.
5. Dr. Khaleda Khanom, Associate Professor, Faculty of Laboratory Medicine, ICMH, Matuail, Dhaka.
6. Dr. Shaikh Badiuzzaman, Assistant Professor, Department of Laboratory Medicine, BSMMU, Dhaka.
7. Dr. Mohammad Mahabubul Hoque, OSD, DGHS, Mohakhali, Dhaka.
*For correspondence
Abstract
Background: Glomerular disorders constitute one of the major causes of morbidity and mortality
in children and adult population. Diagnosing the pattern of glomerular diseases is important as the
treatment and outcome differs in different types. This study was undertaken to find out the various
morphological patterns of glomerular diseases among children at selected tertiary care hospital in
Bangladesh.
Methods: This cross-sectional study was carried out at the Department of Pathology, Bangabandhu
Sheikh Mujib Medical University (BSMMU), Dhaka from the period of September 2014 to June
2016. Renal biopsy samples of children with nephrotic syndrome and nephritic syndrome, children
having asymptomatic haematuria and isolated proteinuria and children presenting with acute and
chronic renal failure were included in this study. For routine histopathological examination, tissue
was preserved in 10% formalin. After completion of routine paraffin processing, hematoxylin and
eosin (H&E) and periodic acid Schiff (PAS) staining were done. For direct immunoflurescence
study, tissue was preserved in normal saline and processed according to standard protocol of
immunoflurescence study. Routine H&E and PAS stained sections of the renal biopsy samples
were examined for changes in glomeruli, tubules, interstitium and blood vessels. The site, pattern
and degree of antibody deposition in the renal tissue were observed by immunoflurescence study.
Final diagnosis was made by correlation of histopathological, immunofluorescence and clinical
findings.
Results: Mesangial proliferative glomerulonephritis (MesPGN) was the most common primary
glomerular disease diagnosed in patients followed by minimal change disease, IgA nephropathy,
membrano-proliferative glomerulonephritis and crescentic glomerulonephritis. Lupus nephritis was
the most common secondary glomerular disease diagnosed in patients.
Conclusion: The reported prevalence of different morphological patterns of glomerular diseases
among children in Bangladesh seems to be no definite pattern. In fact, the patterns vary according
to the geographical area, environment and racial background.
[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):80-88]
Keywords: Glomerular diseases among children, Light and immunoflurescence microscopy, Tertiary care hospitals.
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