Author: bapath

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jhc2025v9i2s5

Journal of Histopathology and Cytopathology

Official Organ of Bangladesh Academy of Pathology

Vol 9, No 2, July 2025

Role of WT1 Immunoexpression in Colorectal Adenocarcinoma: A Study in a Tertiary Care Hospital

*Nupur FP,1 Jeba R,2 Khan ZB,3 Karim SS,4 Zabin SG,5 Khan RR,6 Dewan RK,7 Jinnah MS8

  1. *Dr. Farjana Pervin Nupur, MBBS, MD (Pathology), Pathologist, Dhaka Medical College, Dhaka, pervin_farjana@yahoo.com
  2. Ruksana Jeba, MBBS, MD (Pathology), Professor and head, Department of pathology, Dhaka Medical College, Dhaka,ruksanajeba67@gmail.com
  3. Zubaida Bahroon Khan, MBBS, MD (Pathology), Associate professor, Department of pathology, Dhaka Medical College, Dhaka, zubaida@gmail.com
  4. Saiyeda Sinthia Karim, MBBS, MD (Pathology), Associate professor, National Institute of Laboratory Medicine and Referral centre, Dhaka, sinthiakarim330@gmail.com
  5. Shirin Gull Zabin, MBBS, MD (Pathology), Assistant professor, Department of pathology, Dhaka Medical College, Dhaka, gullzabin23@gmail.com
  6. Rizwana Rahman Khan, MBBS, MD (Pathology), Assistant professor, Department of pathology, Dhaka Medical College, Dhaka, drizwanasbmc@gmail.com
  7. MD. Rezaul Karim Dewan, MBBS, MD (Pathology), Professor and head, Department of Pathology, Greenlife Medical College Hospital, Dhaka, rezadewan22@gmail.com
  8. Mohammed Shahed Ali Jinnah, MBBS, MD (Pathology), Professor and ex-director, National Institute of Laboratory Medicine & Referral Centre, Dhaka, shahed.jinnah63@gmail.com

* For correspondence
Abstract
Background: Wilms Tumor 1 (WT1) is a transcription factor involved in cellular proliferation and differentiation, with emerging relevance in various malignancies including colorectal adenocarcinoma (CRC). The aim of this study was to assess the immunohistochemical expression of WT1 in colorectal adenocarcinoma and determine its association with age, gender, histological type, grade, stage, lymphovascular invasion (LVI), and perineural invasion (PNI).
Methods:This cross-sectional observational study included 97 histopathologically diagnosed cases of colorectal adenocarcinoma, selected using purposive sampling. The study was conducted at the Department of Pathology, Dhaka Medical College Hospital, while WT1 mmunohistochemistry was performed at the Immunohistochemistry Laboratory of Bangladesh Medical University, Dhaka. The study duration was from March 2021 to February 2025. Qualitative data were expressed as frequency and percentage. The Chi- square and Fisher’s exact test were used to assess the relation    between qualitative variables. A p-value less than 0.05 were considered as significant.
Results:Among 97 cases, 55% showed low and 45% showed high WT1 expression. High WT1 expression was significantly associated with mucinous type (p=0.0214), poorly differentiated tumors (p=0.0012), and advanced pathological T (p=0.0007) and N stages (p=0.0003). No significant association was observed with age, gender, LVI, or PNI.
Conclusion: WT1 immunoexpression associates with aggressive histopathological features in colorectal adenocarcinoma, suggesting its potential role as a prognostic marker.

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):97-104]
DOI: https://www.doi.org/10.69950/jhc2025v9i2s5

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jhc2025v9i2s6

Journal of Histopathology and Cytopathology

Official Organ of Bangladesh Academy of Pathology

Vol 9, No 2, July 2025

Histological Variants of Basal Cell Carcinoma in Patients Operated in a Tertiary Level Hospital of Bangladesh: A Retrospective Study

Datta T,1 *Asaduzzaman,2 Ray TK,3 Emita U4

  1. Tanusre 
    1. Tanusree Datta, MBBS, MD (Pathology), Junior Consultant (current charge), Histopathology Department, National Institute of Burn and Plastic Surgery. Dhaka. tanusreebsmmu89@gmail.com
    2. *Dr. Asaduzzaman, MBBS, MD (Pathology), Associate Professor, Histopathology Department, National Institute of Burn and Plastic Surgery. Dhaka. dr.asad37@gmail.com
    3. Tapan Kamar Ray, MBBS, FCPS Trainee Officer, Combined Military Hospital, Dhaka, tapanrmc10@gmail.com
    4. Umama-Tun-Nesa Emita, MBBS, MD (Pathology), Pathologist, Department of Pathology, Khulna Medical College, Khulna. emita.rmc@gmail.com

     

    *For correspondence

Abstract
Introduction: Basal cell carcinoma (BCC) typically affects older persons with photo-exposed areas. Histological classification of BCC is essential for determining the risk of recurrence, assessing the percentage of histological groups, and comparing treatment results.
Aim: This study aimed to determine the frequency of various types of BCCs encountered in our practice, site predilection, age and sex distribution, and histological types that mostly involve the particular age and sex.
Method: This study was conducted in the department of Histopathology, National Institute of Burn and Plastic Surgery, Dhaka. The study was retrospective, observational, and conducted during the period from January 2020 to December 2021 (2 years).
Result: In this study, a total of 31 cases were included. Among them, the most frequent type found was nodular, the majority of cases involved the head and neck region, and were mostly seen in the 6th decade, with female predominance.
Conclusion: In the present study, the majority (93.5%) of the BCC were located on the head and neck region, the average cases was in the 6th decade, mostly found in female patients, and the nodular type of BCC was the most common variant.

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):105-110]
DOI: https://www.doi.org/10.69950/jhc2025v9i2s6

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jhc2025v9i2s7

Journal of Histopathology and Cytopathology

Official Organ of Bangladesh Academy of Pathology

Vol 9, No 2, July 2025

Heterotopic Ossification of the Gallbladder Associated with Chronic Cholecystitis: A case Report

*Anjum R,1 Hossain GZ 2

  1. *Dr. Rahat Anjum, Associate Consultant, Histopathology & Cytopathology, Apollo Imperial Hospitals, Zakir Hossain Road, Chittagong. r.dr@gmail.com
  2. G.M Zakir Hossain, Professor, Department of Urology, Chittagong Medical College Hospital

*For correspondence
Abstract
Heterotopic ossification in the gall bladder is a rare condition, with few reported cases. Its pathogenesis remains unclear, though chronic inflammation may predispose to the condition. An 18-year-old girl underwent laparoscopic cholecystectomy due to persistent upper quadrant pain, nausea, and postprandial vomiting. Preoperative ultrasound revealed a polyp with features of cholecystitis. The post-operative course was uneventful.  Histopathological examination showed fibromuscular hyperplasia, infiltration of chronic inflammatory cells, including foamy histiocytes, in the lamina propria. Rokitansky–Aschoff sinuses were present. The polypoid area exhibited denuded epithelium, mature bone formation, and marrow elements without evidence of metaplasia,  dysplasia or malignancy.

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):111-113]
DOI: https://www.doi.org/10.69950/jhc2025v9i2s7

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