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JHC 2023 July v7 i2 s8

Original Contribution

Role of Reticulin & Masson’s Trichrome Stains in Evaluation of Extent of
Fibrosis in Bone Marrow Biopsies: A Histochemical Study on 36 Cases of
Primary Myelofibrosis

1.*Dr. Sadia Afroz, Medical Officer, Department of Histopathology, National Institute of Cancer Research &
Hospital (NICRH), Mohakhali, Dhaka-1212, Bangladesh; sadiatoma2813@gmail.com
2. Dr. Bishnu Pada Dey, Associate Professor, Department of Pathology, BSMMU.
3. Dr. Rumana Yasmin, Assistant Professor, Department of Pathology, Dhaka Central International Medical College,
Dhaka, Bangladesh.
4. Dr. Evana Kabir, Specialist, Laboratory, United Hospital, Gulshan-2, Dhaka, Bangladesh
5. Dr. Syeeda Shiraj-Um-Mahmuda, Lecturer, Department of Pathology, Dhaka Medical College & Hospital, Dhaka.
6. Dr. Tasmia Islam, Specialist (Pathology), Square Hospital Limited, Dhaka-1205, Bangladesh.
7. Dr. Ummey Salma Shabnam, , Assistant Professor, Department of Histopathology, National Institute of Cancer
Research & Hospital (NICRH), Mohakhali, Dhaka-1212, Bangladesh.
8. Dr. A.K.M. Nurul Kabir, Professor, Department of Pathology, Bangabandhu Sheikh Mujib Medical University
(BSMMU), Shahbagh, Dhaka-1000, Bangladesh.
*For correspondence

Abstract
Background: Primary myelofibrosis (PMF) is a rare hematological malignancy with higher
mortality and morbidity due to progression of bone marrow fibrosis (BMF). The amount of bone
marrow reticulin and collagen fibrosis is detected by reticulin and Masson’s trichrome (MT) stains,
respectively. The study aimed to explore the pattern and degree of BMF in PMF cases with above
mentioned special stains along with conventional Hematoxylin& Eosin (H&E) stain.
Methods: Thirty-six histopathologically suggested or diagnosed cases of PMF were studied at the
Department of Pathology, BSMMU. Paraffin blocks of trephine biopsy specimens of the selected
cases were collected. H&E, reticulin and MT stains were applied on the re-cut sections. Extent of
fibrosis was assessed by examining the sections with each special stain using a proposed four
scaled semiquantitative bone marrow fibrosis (MF) grading systems. The cases were divided into
two groups. Patients having grade MF-0 to MF-1 were considered absent pathological BMF. Those
cases with MF-2 to MF-3 were encountered with present pathological BMF.
Results: In this study, 19 (52.8%) PMF cases had pathological BMF diagnosed by only H&E.
Moreover, thirty four (94.4%) patients had pathological BMF corresponding to MF-2 to MF-3
detected by special stains.
Conclusion: Significantly higher (p<0.05) detection of pathological BMF cases with use of
additional special stains recommends that, both reticulin and MT stains can be used routinely to
assess BMF properly according to special grading system.

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):103-111]
Keywords: Bone marrow fibrosis, Primary myelofibrosis, Reticulin fibrosis, Collagen fibrosis,
Reticulin stain, Masson’s trichrome stain, Hematoxylin & Eosin, Bone marrow fibrosis (MF) grade.

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JHC 2023 v7 i2 s7

Original Contribution

Histomorphological Pattern of Vocal Cord Lesions in a Tertiary Care Hospital

1.*Dr Naila Awal, Assistant Professor, Department of Pathology, Green Life Medical College, Dhaka.
nailaawal@gmail.com
2. Dr Md Saidur Rahman, Professor and Head, Department of Pathology, Sher-e-Bangla Medical College, Barishal.
3. Dr Subrata Ray, Assistant Professor, Department of Pathology, Sher-e-Bangla Medical College, Barishal.
4. Dr Abirvab Naha, Assistant Professor, Department of Otolaryngology- Head and neck Surgery, Bangabandhu
Sheikh Mujib Medical University, Dhaka.
5. Dr Rumana Yasmin, Assistant Professor, Department of Pathology, Dhaka Central International Medical College,
Dhaka.
6. Dr Lyzu Sharmin, Assistant Professor, Histopathology department, National Institute of Cancer Research and
Hospital, Dhaka.
7. Dr Md. Shafiqul Alam, Associate Professor, Radiology and Imaging Department, Monno Medical College,
Manikgonj.
*For correspondence

Abstract
Background: Voice is the most important component for human to communicate their ideas,
emotion and personality with the outside world. Vocal cord lesion is one of the most common
problems in the Otolaryngology Department. Even the smallest swelling on the free edge of vocal
cord can cause voice disturbances and has significant social and psychological impact. The aim of
our study was to find out the frequencies of the vocal cord lesions and to assess the various
histomorphological characteristics of these lesions among the patients of various age groups in
Bangladeshi population.
Methods: A retrospective, record review (secondary data analysis) study was carried out among
2054 patients who underwent surgery in Green Life hospital for any vocal cord lesion between the
period of 2011 to 2021. The data was collected from an electronic database from Histopathology
Department of Green Life Hospital.
Result: The study population consists of total 2054 cases with male and female ratio of 3.8:1. The
age of the patient was between 5 to 95 years and the majority of the patients (27%) were presented
with 3rd to 4th decades. Male (79%) were commonly affected than female (21%). Male patients
showed higher percentage in 40-50 years age group (21%), while female patient showed higher
percentage in 18-40 years age group (13%).
A total of 63 (3.1 %) cases were non neoplastic, 1966 (95.7%) cases were neoplastic, and 25 (1.2
%) cases were diagnosed as premalignant. The most common vocal cord lesion was vocal cord
polyp (81.3%) followed by invasive squamous cell carcinoma 272 cases (13.2%).
Conclusion: The histopathological examination of vocal cord lesion is the gold standard diagnostic
tool to evaluate voice disorders. The early diagnosis should be done carefully to evaluate
underlying pathology for better management

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):95-102]
Keywords: Vocal cord, Histopathology

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JHC 2023 July v7 i2 s6

Original Contribution
Use of Sentinel Lymph Node Biopsy to Detect Metastatic Spread in Axillary Nodes in Primary Breast Carcinoma

1.*Dr. SM. Basitur Roshid, Assistant Professor, Department of Pathology, BIRDEM, Dhaka,
dr.basitroshid@gmail.com
2. Professor Mohammed Kamal, Ex Chairman, Department of Pathology, BSMMU, Dhaka.
3. Dr. Mohammad Mahabubul Hoque, Assistant Professor of Pathology, National Institute of Laboratory Medicine
and Referral Centre, Agargaon, Dhaka. drshaikat331@gmail.com
4. Dr. Muhammad Ariful Haque, Assistant Professor, Faculty of Laboratory Medicine, ICMH, Matuail, Dhaka
*For correspondence

Abstract
Objectives: Although sentinel lymph node (SLN) biopsy is recommended approach in the evaluation
of metastatic status of lymph node in breast cancer management, the efficacy of this technique require
validation. In current study, results of patients who underwent sentinel lymph node biopsy by using
only methylene blue dye were evaluated.
Methods: The study included 17 cases of primary ductal carcinoma with tumor size up to 5.0cm.
Patients who had previous breast or axilla surgery and radiation or chemotherapy for any breast lesion
were excluded from this study. During operation 3.0 ml of sterilized methylene blue dye were
injected in peritumoral area or around the lumpectomy cavity. The axillary sentinel lymph node was
removed and later on axillary dissection was done. Imprint cytology, frozen section and routine H&E
slides were made from sentinel lymph node.
Results: The sentinel lymph node was found in all cases. Among them, eight (47.1%) cases were
positive for SLN and axillary lymph node and nine (52.9%) cases were negative for both SLN and
axillary lymph node. No false negative result was observed and no complication was recorded due to
use of methylene blue dye.
Conclusion: Sentinel lymph node biopsy is a safe and accurate method in staging the early breast
cancer. This method will reduce the unnecessary axillary lymph node dissection and other morbidity
associated with it

[Journal of Histopathology and Cytopathology, 2023 Jul; 7 (2):89-94]
Keywords: Breast cancer, Sentinel lymph node biopsy, Methylene blue dye

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