Tag: Astrocytoma

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jhc.2026.10.1.5

Journal of Histopathology and Cytopathology
Official Journal of Bangladesh Academy of Pathology
Vol 10, No 1, January 2026

Expression of FLI1 in Astrocytoma and its Association with Histological Parameters
*Sultana S,1 Khan ZB,2 Rahiduzzaman SM,3 Doly NJ,4 Tahsin KN,5  Jannat MR,6 Ahmed S,7 Kashfi SK8

Abstract
Background: Astrocytomas represent the most prevalent type of glial tumor. This category encompasses a diverse array of neoplasms that vary in their central nervous system (CNS) locations, morphological characteristics, and patterns of progression and invasiveness. FLI1, a nuclear transcription factor, plays a significant role in enhancing cellular proliferation and tumorigenesis, thereby serving as a prognostic indicator for numerous human tumors.
Objective: To see FLI1 expression in astrocytoma and its association with histological parameters (cellularity, nuclear atypia, mitosis, microvascular proliferation and necrosis ) of astrocytic tumors.
Methods: It was a cross-sectional observational study conducted with 50 samples selected using purposive sampling technique at the Department of Pathology of Dhaka Medical College. The samples were drawn from histomorphologically diagnosed astrocytoma cases within the period from March 2022 to February 2024. The collected 50 paraffin blocks were sectioned, stained with hematoxylin and eosin (H&E). Immunostaining with FLI1 was done in all cases. Relevant information were collected and recorded in a predesigned data sheet. Statistical analysis was carried out as required.
Results: Among total 50 cases, the patients’ ages ranged from 4 to 92 years, with a mean age of 37.8 ±19.9 years. The male-to-female ratio was 1.5:1. The most common tumor location was in the frontal lobe (28.0%), approximately half showing moderate cellularity (48.0%), moderate atypia (36.0%), mitosis (56.0%), and necrosis (32.0%). Among total 50 cases grade IV tumor was 36% followed by Grade I tumors (22.0%) and Grade II tumors (22.0%). The majority of cases exhibited high expression (FLI1 score ≥4) at 74.0%, while the remaining cases showed low expression (FLI1 score ≤3) at 26.0%. FLI1 expression was associated with atypia (p < 0.001), mitosis (p < 0.001), and necrosis (p= 0.029). The calculated p-value (<0.001) emphasizes the statistical significance of this association.
Conclusion: The findings of this study suggest that elevated expression of FLI1 is positively correlated with atypical features, mitotic activity, and necrosis in astrocytoma. Assessing FLI1 may serve as a predictive indicator for tumor advancement and a prognostic marker for individuals diagnosed with astrocytoma.

[Journal of Histopathology and Cytopathology, 2026 Jan; 10 (1):38-48]
DOI: https://www.doi.org/10.69950/jhc.2026.10.1.5
Keywords: Astrocytoma, FLI1 expression, Histological parameters.

  1. *Dr. Shahana Sultana, MBBS, MD (Pathology), Assistant Professor, Green life Medical College, Dhaka, shahanashova935@gmail.com
  2. Dr. Dr. Zubaida Bahroon Khan, MBBS, MD (Pathology), Associate Professor, Department of Pathology, Dhaka Medical College, Dhaka
  3. Dr. S.M. Rahiduzzaman, MBBS, MD (Cardiology), Resident, Sir Salimullah medical college, Dhaka. rahid39rpmc@gmail.com
  4. Dr. Nusrat Jahan Doly, MBBS, MD (Pathology), Assistant Surgeon, Department of Histopathology, National Institute of Burn and Plastic Surgery, Dhaka, doctornusratjahan1990@gmail.com
  5. Dr. Kazi Nafisa Tahsin, MBBS, MD (Pathology), Assistant Surgeon, Department of Histopathology, National Institute of Burn and Plastic Surgery, Dhaka, kazinafisa29@gmail.com
  6. Dr. Mst. Rubeyatul Jannat, MD pathology, Assistant professor, Department of Pathology, Dr.Sirajul Islam medical College, Dhaka, rubeyatulj@gmail.com
  7. Dr. Shamim Ahamed, MD (Pathology), Assistant Professor, Department of Pathology, Brahmanbaria Medical College, Brahmanbaria, shawonahamedd@gmail.com
  8. Dr. Sayeed Kishwara Kashfi, MD (Pathology), Assistant Professor, Department of Pathology, Ahsania Mission Medical college, Dhaka, kishwarakashfi06@gmail.com 

*For correspondence
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jhc.2026.10.1.4

Journal of Histopathology and Cytopathology

Official Journal of Bangladesh Academy of Pathology
Vol 10, No 1, January 2026
Expression of FLI1 in Astrocytoma and its Association with WHO Grade
*Sultana S,1 Rahiduzzaman SM,2 Yeamin MA,3 Rashid HO,4 Akter J,5 Islam M,6 Jeba R7
Abstract
Background: Astrocytoma is one of the most common glial tumors of the central nervous system. Prognosis and treatment modalities for astrocytoma vary according to their grades. Understanding the biological behavior of tumors and their sensitivity to chemotherapy or radiotherapy is difficult through histopathological grading alone. The transcription factor Friend leukemia virus integration 1 (FLI1) is abnormally expressed in astrocytoma and is associated with tumor grade.
Objective: To see FLI1 expression in astrocytoma and its association with WHO grade.
Methodology: It was a cross-sectional observational study conducted with 50 samples selected using purposive sampling technique at the Department of Pathology of Dhaka Medical College. The samples were drawn from histologically diagnosed astrocytoma cases within the period from March 2022 to February 2024. These samples were categorized into histopathological grades according to the WHO classification 2016. The collected 50 paraffin blocks were sectioned, stained with hematoxylin and eosin (H&E). Immunostaining with FLI1 was done in all cases. Relevant information was collected and recorded in a predesigned data sheet. Statistical analysis was carried out as required.
Results: Among total 50 cases, the patients’ ages ranged from 4 to 92 years, with a mean age of 37.8 ± 19.9 years. The male-to-female ratio was 1.5:1. Among total 50 cases grade IV tumor was 36% followed by Grade I tumors (22.0%) and Grade II tumors (22.0%). The majority of cases exhibited high expression (FLI1 score ≥4) at 74.0%, while the remaining cases showed low expression (FLI1 score ≤3) at 26.0%. Grade I and II tumors mostly displayed low FLI1 expression, whereas grade III and IV tumors predominantly exhibited high FLI1 expression. Among the cases with high FLI1 expression, the majority were grade IV tumors (45.9%), followed by grade III tumors (27.0%). Conversely, among the cases 26% with low FLI1 expression, the majority were grade I (46.2%) and grade II tumors (46.2%). The calculated p-value (<0.001) emphasizes the statistical significance of this association.
Conclusion: The results of this study indicate positive association of FLI1 expression with WHO grade in astrocytoma. Low expression of FLI1 was observed in low- grade astrocytomas and high expression of FLI1 was observed in high-grade astrocytoma.

[Journal of Histopathology and Cytopathology, 2026 Jan; 10 (1):27-37]
DOI: https://www.doi.org/10.69950/jhc.2026.10.1.4
Keywords: Astrocytoma, FLI1 expression, WHO Grade.

  1. *Dr. Shahana Sultana, MBBS, MD (Pathology), Assistant Professor, Green Life Medical College, Dhaka, shahanashova935@gmail.com,
  2. Dr. S.M. Rahiduzzaman, MBBS, MD (Cardiology), Resident, Sir Salimullah Medical College, Dhaka. rahid39rpmc@gmail.com
  3. Dr. Dr. Md. Abdullah Yeamin, MBBS, MD (Pathology),Lecturer, Jashore Medical College, Jashore. peyal4794@gmail.com
  4. Dr, Harun Or Rashid, MBBS, MD (Pathology), Lecturer, Shaheed M Monsur Ali Medical College, Sirajgonj. drharunorrashidjewel@gmail.com
  5. Dr. Dr. Jhuma Akter, MBBS, MD (Pathology), Pathologist, Dhaka Medical College, Dhaka, jhuma.kmc@gmail.com
  6. Dr, Mainul Islam, MBBS, MD (Pathology), Assistant Professor, Department of Pathology, Dhaka Medical College, Dhaka. mainul.62.34@gmail.com
  7. Dr. Ruksana Jeba, MBBS, MD (Pathology), Professor and head, Department of Pathology, Dhaka Medical College, Dhaka, ruksanajeba67@gmail.com

 

*For correspondence

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jhc.2024.8.1.04

Journal of Histopathology and Cytopathology

 January 2024 Volume 8 Issue 1

Original Contribution

Immunoexpression of WT1 in Astrocytoma and its Correlation with Histopathological Grade

1. *Dr. Evana Kabir, Specialist (Pathology), United Hospital Limited, Gulshan-2, Dhaka, Bangladesh; evana19901@gmail.com
2. Professor Dr. Md Rezaul Karim Dewan, Professor, Department of Pathology, Greenlife Medical College, Ex-Professor and head, Dhaka Medical College.
3. Dr. Rejwana Nahar, Assistant Professor, Department of Pathology, Mugda Medical college, Dhaka.
4. Dr. Sadia Afroz, Medical Officer, Department of Histopathology, National Institute of Cancer Research & Hospital (NICRH), Mohakhali, Dhaka-1212, Bangladesh.
5. Dr. Shamim Rahman, Assistant Professor, Department of Pathology, Jahurul Islam Medical College, Bajitpur Bangladesh.
6. Dr. Tasmia Islam, Specialist (Pathology), Square Hospital Limited, Sher-e-Bangla Nagar, Dhaka-1205, Bangladesh.
7. Dr. Khondoker Rakibul Hoque, Senior House Officer, Department of Orthopaedics, United Hospital Limited, Gulshan, Dhaka.

*For correspondence.
Abstract
Background:  Astrocytoma comprises a group of neoplasms that differ in their location in the Central nervous system, morphologic features and invasive behavior. These are therapeutically challenging for their infiltrative growth pattern, occasionally resistant to conventional therapies and invariably high grade tumors have shown poor prognosis. Moreover biological behavior of astrocytoma and chance of recurrence cannot be ruled out by histopathological evaluation alone. So, new predictive marker for determining tumor progression is at stake.
Objectives: The purpose of the study was to evaluate WT1 expression in astrocytoma and its correlation with histological grade.
Methods:  The cross-sectional observational study was conducted in the Department of Pathology, Dhaka Medical College (DMC) from September 2019 to August 2021. Histologically diagnosed 51 cases of different grades of astrocytoma were included in this study. Immunostaining with WT1 protein was done in all cases. The data were collected and statistical analysis was done by SPSS.
Results: The mean age for grade I, II, III and IV astrocytoma’s are 14, 27.5, 38.6 and 44 years respectively. There were 30 male and 21 female patients with the ratio 1.42:1. Among 51 cases of astrocytoma’s Grade I, II ,III and IV are 10, 15, 8 and 18 in number. By Immunohistochemical study positive WT1 expression was seen in all cases of astrocytoma’s (100%). Out of 25 cases of low grade astrocytoma’s (WHO grade I and II), expression was found mild in 12 (48%) cases, moderate in 11 (54%) cases and marked only 02 (8%) cases. Regarding 26 cases of high grade astrocytoma’s (WHO grade III and IV) expression found mostly marked in 16 (61.5%) cases, moderate in 09 (34.7%) and mild only in 01 (3.8%) cases. These data shows WT1 expression increases with WHO tumor grades and significant positive (p<0.001) correlation between WT1 expression and tumor grade.
Conclusion: The study reveals positive correlation between WT1 expressions with WHO tumor grade in astrocytoma. Furthermore, evaluation of WT1 with histopathological grading may provide information about tumor progression as well as guide the clinicians for therapeutic purposes.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):19-30]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.04
Keywords: Astrocytoma, tumor grade, WT1 expression, tumor progression, Glioblastoma

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