Tag: Immunohistochemistry

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jhc2025v9i1s2

Original Article

Association of E-cadherin Expression with Histopathological Grades and Clinical Stages of Laryngeal Squamous Cell Carcinoma

*Tayeb M,1Mimi SA,2 Kamal MS,3 Snigdha SS4

  1. *Dr. Mohammad Tayeb, MD (Pathology), Assistant Professor, Department of Pathology, Jahurul Islam Medical College. tmohammad75@yahoo.com
  2. Professor Dr. Shamim Akhter Mimi, M. Phil (Pathology), Professor and Head, Department of Pathology, Sylhet MAG Osmani Medical College.
  3. Mohammad Shah Kamal, FCPS (ENT), Associate Professor, Department of Otorhinology & Head-Neck Surgery, Sylhet MAG Osmani Medical College.
  4. Shyla Sharmin Snigdha, MD (Pathology),Assistant professor, Department of Pathology, Zainul Haque Sikder Women’s Medical College.

*For correspondence

Abstract
Background: E-cadherin gene plays an important role in the carcinogenesis of laryngeal squamous cell carcinoma (LSCC). This study aimed to evaluate the immunohistochemical expression of E-cadherin in different grades and clinical stages of LSCC and to determine the association of E-cadherin with clinical stages and histopathological grades.
Methods: This cross-sectional study was conducted in the Department of Pathology, Sylhet MAG Osmani Medical College, from March 2021 to January 2023. Immunohistochemistry was performed in 50 histopathologically diagnosed cases of LSCC using a commercially available anti-E-cadherin antibody. The total score of immunoreaction was calculated by multiplying the expression score and intensity score.
Results: The mean age of the LSCC patients was 62.8 years, and 66% were male. Grade I is the most frequent histopathological grade (48%), followed by grade II (42%) and grade III (10%). The most frequent clinical T stage was T3 (56%), and the N stage was N2 (58.06%). E-cadherin expression was positive in 72% of cases; the rest, 28%, showed reduced expression. A significant association was found between E-cadherin expression with histopathological grades (p=0.007) and clinical N stage (p=0.009) but not significant with clinical T stage (p=0.502), anatomic site (P=0.132), and the habit of smoking (0.276).

Conclusion: LSCC patients with reduced E-cadherin expression are at the risk of high-grade carcinoma and nodal metastasis. So, the expression of E-cadherin in pre-treatment biopsy samples can be utilized as one of the prognostic factors and advocated to anti-E-cadherin targeted therapy in LSCC.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):3-9]

Keywords: E-cadherin, Expression, Immunohistochemistry, laryngeal squamous cell carcinoma.

DOI: https://www.doi.org/10.69950/jhc2025v9i1s2

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jhc.2024.v8.i2.5


Editorial
Original Contribution
Correlation of Ki-67 Proliferating Index with Histological Types and Characterization of Mucin in Colorectal Carcinoma
*Sultana S,1 Islam N,2 Kabir E3

  1. *Dr. Sahela Sultana, MBBS. MD, Assistant Professor, Department of Pathology, Ibrahim Medical College, Dhaka. sultana.sahela83@gmail.com
  2. Dr. Nasimul Islam. MBBS. M. Phil., Professor & Head, Department of Pathology, Anwar Khan Modern Medical College & Hospital
  3. Dr. Enamul Kabir. MBBS. M.Phil. MSc(Path), Professor, Department of Pathology, Popular Medical College, Dhaka

*For Correspondence
Abstract
Background: Colorectal carcinoma is a major cause of cancer associated with a high rate of morbidity and mortality in the western world. One of the pathologic features considered to be important for prognosis is mucin production. Many authors confirmed that colon carcinomas with high mucin content tend to recur locally and carry a poor prognosis.
Aim: Correlation of Ki-67 proliferating index with different type of colorectal carcinoma as well as characterization of mucin.
Method: This cross sectional study was conducted at Sir Salimullah Medical College, Department of pathology   from July 2014 to June 2016. Ninety eight patients with colorectal carcinoma was enrolled in this  study  who underwent surgical resection of colon, adenocarcinomas. For histological classification  we used the WHO recommendation (2000) and to be more accurate we sub-classified mucinous adenocarcinomas by morphometrical  analysis in three categories: pure mucinous, with extracellular mucin more than 80% of the tumoral volume; mixed type, with 50–80%  extracellular mucin; and mixed type with less than 50% extracellular mucin and their  correlation with Ki-67 proliferating index . For histochemical investigation, we used stains such as: D- PAS and Alcian Blue. A technique of manual tissue array was employed to see Ki-67 expression by IHC method. Ki-67 is a proliferation associated nuclear antigen which can be recognized by MIB-1 monoclonal antibody.
Result: It was observed that Ki-67 labeling index was high in nonmucinous tumor  compared to mucinous adenocarcinoma and signet ring cell carcinoma which is  statistically significant (P<0.05). Histochemical stain of mucin where both D-PAS and Alcian Blue positive cases(mixed type)  are more than the Only D-PAS positive cases(pure type). Ki-67 proliferating index was also high in mixed type mucinous adenocarcinoma (<50%) compared to pure (>80%) and mixed type (50-80%). The result was statistically significant (p<0.05). Correlation of Ki-67 proliferating index with histologic type as well as mucin characterization and thereby provide information to clinician to better understanding  of the  treatment as well as prognosis.

[Journal of Histopathology and Cytopathology, 2024 Jul; 8 (2):100-108]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i2.5
Keywords: Colorectal carcinoma, Mucin, Immunohistochemistry, Ki-67.
Full article

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jhc.2024.8.1.05

Journal of Histopathology and Cytopathology
January 2024 Volume 8 issue 1

Original Contribution

Evaluation of Pediatric Common Solid Small Round Cell Tumors: An Immunohistochemical Study

1. Dr. Md. Shahrior Nahid, MBBS, MD(Pathology), Resident Medical Officer, Department of Pathology, National Institute of Laboratory Medicine and Referral Centre (NILMRC), Dhaka-1207. shahrior.nahid@gmail.com
2. Processor (Dr.) Ferdousy Begum, MBBS, MD (Pathology), Professor and Ex-Chairman, Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
3. Professor (Dr.) Mohammed Shahed Ali Jinnah, MBBS, MD(Pathology), Professor of Pathology, Director, National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207.
4. Dr. Umama-Tun-Nesa Emita, MBBS, MD(Pathology), Pathologist, Khulna Medical College Hospital, Khulna.
5. Dr Md. Mahabub Alam, MBBS, MD (Pathology), Assistant Professor (Current Charge), Bashundhara Ad-din Medical College, Dhaka.
6. Dr. Arbin Siddiquea, MBBS, MD (Biochemistry), Lecturer, Department of Biochemistry, Shaheed Suhrawardy Medical College, Dhaka-1207.
7. Dr. Tasmina Anam, MBBS, MPhil (Immunology), Medical Officer, Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
⃰ For correspondence

Abstract
Background: Pediatric small round cell tumors (SRCTs) are diagnostically challenging lesions due to their primitive character.  With the rising incidence and having better treatment outcome as compared to the past, the categorization of SRCTs into definitive histological types is extremely important as individual tumor differs therapeutically and has separate prognostic significance. Immunohistochemistry (IHC) can play an important role here.
Objective: The present study was designed to evaluate the role of immunohistochemistry (IHC) in the differential diagnoses of pediatric SRCTs.
Results: In this study, various histomorphological types of pediatric SRCTs were identified in about 97% of cases with the aid of immunohistochemical stains. However, about 3% of cases remain unclassified even after immunohistochemical tests. The different morphological patterns were as follows; 24.4% rhabdomyosarcoma, 22.2% lymphoblastic lymphoma, 22.2% neuroblastoma, 22.2% soft tissue Ewing sarcoma, 4.44% Wilms Tumor, and 1.48% poorly differentiated synovial sarcoma.
Conclusion: Immunohistochemistry plays an important role as is evident from the present study and supported by many previous studies in categorizing undifferentiated or poorly differentiated small round cell tumors of childhood.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):31-40]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.05
Keywords: Small round cell tumors (SRCTs), Immunohistochemistry (IHC)

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