Tag: Immunohistochemistry

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jhc.2024.v8.i2.5


Editorial
Original Contribution
Correlation of Ki-67 Proliferating Index with Histological Types and Characterization of Mucin in Colorectal Carcinoma
*Sultana S,1 Islam N,2 Kabir E3

  1. *Dr. Sahela Sultana, MBBS. MD, Assistant Professor, Department of Pathology, Ibrahim Medical College, Dhaka. sultana.sahela83@gmail.com
  2. Dr. Nasimul Islam. MBBS. M. Phil., Professor & Head, Department of Pathology, Anwar Khan Modern Medical College & Hospital
  3. Dr. Enamul Kabir. MBBS. M.Phil. MSc(Path), Professor, Department of Pathology, Popular Medical College, Dhaka

*For Correspondence
Abstract
Background: Colorectal carcinoma is a major cause of cancer associated with a high rate of morbidity and mortality in the western world. One of the pathologic features considered to be important for prognosis is mucin production. Many authors confirmed that colon carcinomas with high mucin content tend to recur locally and carry a poor prognosis.
Aim: Correlation of Ki-67 proliferating index with different type of colorectal carcinoma as well as characterization of mucin.
Method: This cross sectional study was conducted at Sir Salimullah Medical College, Department of pathology   from July 2014 to June 2016. Ninety eight patients with colorectal carcinoma was enrolled in this  study  who underwent surgical resection of colon, adenocarcinomas. For histological classification  we used the WHO recommendation (2000) and to be more accurate we sub-classified mucinous adenocarcinomas by morphometrical  analysis in three categories: pure mucinous, with extracellular mucin more than 80% of the tumoral volume; mixed type, with 50–80%  extracellular mucin; and mixed type with less than 50% extracellular mucin and their  correlation with Ki-67 proliferating index . For histochemical investigation, we used stains such as: D- PAS and Alcian Blue. A technique of manual tissue array was employed to see Ki-67 expression by IHC method. Ki-67 is a proliferation associated nuclear antigen which can be recognized by MIB-1 monoclonal antibody.
Result: It was observed that Ki-67 labeling index was high in nonmucinous tumor  compared to mucinous adenocarcinoma and signet ring cell carcinoma which is  statistically significant (P<0.05). Histochemical stain of mucin where both D-PAS and Alcian Blue positive cases(mixed type)  are more than the Only D-PAS positive cases(pure type). Ki-67 proliferating index was also high in mixed type mucinous adenocarcinoma (<50%) compared to pure (>80%) and mixed type (50-80%). The result was statistically significant (p<0.05). Correlation of Ki-67 proliferating index with histologic type as well as mucin characterization and thereby provide information to clinician to better understanding  of the  treatment as well as prognosis.

[Journal of Histopathology and Cytopathology, 2024 Jul; 8 (2):100-108]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i2.5
Keywords: Colorectal carcinoma, Mucin, Immunohistochemistry, Ki-67.
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jhc.2024.8.1.05

Journal of Histopathology and Cytopathology
January 2024 Volume 8 issue 1

Original Contribution

Evaluation of Pediatric Common Solid Small Round Cell Tumors: An Immunohistochemical Study

1. Dr. Md. Shahrior Nahid, MBBS, MD(Pathology), Resident Medical Officer, Department of Pathology, National Institute of Laboratory Medicine and Referral Centre (NILMRC), Dhaka-1207. shahrior.nahid@gmail.com
2. Processor (Dr.) Ferdousy Begum, MBBS, MD (Pathology), Professor and Ex-Chairman, Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
3. Professor (Dr.) Mohammed Shahed Ali Jinnah, MBBS, MD(Pathology), Professor of Pathology, Director, National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207.
4. Dr. Umama-Tun-Nesa Emita, MBBS, MD(Pathology), Pathologist, Khulna Medical College Hospital, Khulna.
5. Dr Md. Mahabub Alam, MBBS, MD (Pathology), Assistant Professor (Current Charge), Bashundhara Ad-din Medical College, Dhaka.
6. Dr. Arbin Siddiquea, MBBS, MD (Biochemistry), Lecturer, Department of Biochemistry, Shaheed Suhrawardy Medical College, Dhaka-1207.
7. Dr. Tasmina Anam, MBBS, MPhil (Immunology), Medical Officer, Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka-1000.
⃰ For correspondence

Abstract
Background: Pediatric small round cell tumors (SRCTs) are diagnostically challenging lesions due to their primitive character.  With the rising incidence and having better treatment outcome as compared to the past, the categorization of SRCTs into definitive histological types is extremely important as individual tumor differs therapeutically and has separate prognostic significance. Immunohistochemistry (IHC) can play an important role here.
Objective: The present study was designed to evaluate the role of immunohistochemistry (IHC) in the differential diagnoses of pediatric SRCTs.
Results: In this study, various histomorphological types of pediatric SRCTs were identified in about 97% of cases with the aid of immunohistochemical stains. However, about 3% of cases remain unclassified even after immunohistochemical tests. The different morphological patterns were as follows; 24.4% rhabdomyosarcoma, 22.2% lymphoblastic lymphoma, 22.2% neuroblastoma, 22.2% soft tissue Ewing sarcoma, 4.44% Wilms Tumor, and 1.48% poorly differentiated synovial sarcoma.
Conclusion: Immunohistochemistry plays an important role as is evident from the present study and supported by many previous studies in categorizing undifferentiated or poorly differentiated small round cell tumors of childhood.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):31-40]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.05
Keywords: Small round cell tumors (SRCTs), Immunohistochemistry (IHC)

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jhc.2024.8.1.03

Journal of Histopathology and Cytopathology

January 2024 Volume 8 Issue 1

Original Contribution

Evaluation of Immunohistochemical Expression of p53 in Colorectal Carcinoma

1. *Dr. Mahfuza Jebun  Mouri; Lecturer, Department of Pathology, Shaheed Suhrawardy Medical College, Sher-E-Bangla Nagar, Dhaka.  mouri@gmail.com.
2. Professor Dr. Enamul Kabir, Professor, Department of Pathology, Popular Medical College, Dhaka.
3. Professor Dr. Shahnaj Begum, Professor, Department of Pathology, Sir Salimullah Medical College, Dhaka.

*For correspondence
Abstract
Background: The most common gastrointestinal malignancy is colorectal carcinoma and is a major cause of morbidity and mortality. In colorectal carcinoma the most frequently mutated gene is p53 tumor suppressor gene. Mutation of p53 gene gives rise to abnormal protein which can be easily detected by immunohistochemistry. Expression of mutant p53 protein has been associated with poor clinical outcome and increased risk of death due to increased aggressiveness of the disease.
Objective: The aim of the study was to see the clinicopathological correlation of mutant p53 expression in colorectal carcinoma.
Method: Total 50 paraffin embedded tissue blocks of histopathologically diagnosed cases of colorectal cancer were evaluated by immunohistochemical staining for mutant p53 expression. The study was performed in Sir Salimullah Medical College, Dhaka (from March, 2018 to February, 2020).
Results: Out of 50 patients studied, 29 cases (58%) expressed mutant p53 protein in the nucleus of malignant cells. There was significant association between  p53  protein expression and clinicopathologic variables such as age (<40 years vs >40 years, p=0.032), site of tumor (left vs right colon, p=0.028), pathological type (mucinous vs non mucinous, p=0.039), grade (a greater tendency towards poor differentiation, p= 0.039), advanced stage (both TNM and Dukes), whereas no significant association was found between mutant p53 protien expression and other parameters like gender and morphological types.
Conclusion: The results of this current study revealed that mutant p53 positive colorectal cancer tended to be related to a higher grade of malignancy, advanced tumor stage and mucinous morphology. The results of this current study revealed that mutant p53 positive colorectal cancer tended to be related to a higher grade of malignancy, advanced tumor stage and mucinous morphology. So, p53 is an important immunohistochemical marker for colorectal cancer patients.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):10-18]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.03
Keywords: Colorectal cancer, p53, Immunohistochemistry
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