Tag: tumor progression

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jhc2025v9i2s2

Journal of Histopathology and Cytopathology

Official Organ of Bangladesh Academy of Pathology

Vol 9, No 2, July 2025

Immune Expression of p53 and Its Association with Histological Risk Classification in Wilms Tumor

*Akter J,1 Jeba R,2 Karim SS,3 Islalm MJ,4 Shahana Sultana,5Yeamin MA,6 Rashid HO,7 Nupur FP,8 Khatun J9

  1. *Dr. Jhuma Akter, MBBS, MD (Pathology), Pathologist, Dhaka Medical College, Dhaka. jhuma.kmc@gmail.com
  2. Ruksana Jeba, MBBS, MD (Pathology),Professor and head, Department of pathology, Dhaka Medical College, Dhaka,ruksanajeba67@gmail.com
  3. Saiyeda Sinthia Karim, MBBS, MD (Pathology), Associate professor, National Institute of Laboratory Medicine and Referral centre, Dhaka, sinthiakarim330@gmail.com
  4. Mohammad Jahirul Islam, Junior consultant, Department of Surgery, Shaheed Suhrawardy Medical College and Hospital, dr.jahir2020@gmail.com
  5. Shahana Sultana, MBBS, MD (Pathology), Assistant Professor (cc), Green life Medical College, Dhaka, shahanashova935@gmail.com
  6. Md. Abdullah Yeamin, MBBS, MD (Pathology),Lecturer, Jashore Medical College, Jashore, peyal4794@gmail.com
  7. Harum Or Rashid, MBBS, MD (Pathology),Lecturer, Shaheed M Monsur Ali Medical College, Sirajgonj, drharunorrashidjewel@gmail.com
  8. Farjana Pervin Nupur, MBBS, MD (Pathology), Lecturer, Pathology, Dhaka Medical College, Dhaka,    pervin_farjana@yahoo.com
  9. Julekha Khatun, MBBS, MD (Pathology), Lecturer, Pathology, Dhaka Medical College, Dhaka, julekhacmc48@gmail.com

* For correspondence

Abstract
Background: Wilms’ tumor, a type of kidney cancer that occurs in children. The p53 gene serves a crucial function as a tumor suppressor. Mutations in p53 are closely linked to the emergence of anaplastic features, which are notably aggressive and suggest an unfavorable prognosis.
Methods: This study was conducted at the Department of Pathology, Dhaka Medical College. A total 30 samples were collected from Dhaka Medical College and a private laboratory. The histological assessments were conducted at the same institutes. Immunohistochemistry was conducted at the Private Diagnosis Centre. Qualitative data were expressed as frequency and percentage. Fisher’s exact test was used to examine the relation between qualitative variables. A p-value less than 0.05 was considered significant.
Results: The age of the patients ranged from 5 months to 14 years, with a mean of 4.9 ± 3.4 years. The male-to-female ratio was 1:1.1. The mean size of the tumor was 8.5 ± 3.4 cm. Tumor laterality varied, with 40.0% on the left, 36.7% on the right, and the remaining 20.0% was not available. Histologically, 83.3% were favorable, and 16.7% were unfavorable. Risk classification showed that 33.3% were low-risk, 50.0% intermediate-risk, and 16.7% high-risk. P53 expression was observed in 23.3% of cases. Favorable histology was associated with negative p53 (100.0%), unfavorable histology with positive p53 (71.4%). Low-risk histology was linked to negative p53 (43.5%), intermediate risk showed mixed patterns (28.6% positive, 56.5% negative), and high-risk histology strongly correlated with positive p53 (71.4%).
Conclusion: The presence of positive p53 protein in Wilms’ tumor could serve as a marker for high-risk tumors as it is associated with an unfavorable prognosis.

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):70-77]
DOI: https://www.doi.org/10.69950/jhc2025v9i2s2
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jhc.2024.8.1.04

Journal of Histopathology and Cytopathology

 January 2024 Volume 8 Issue 1

Original Contribution

Immunoexpression of WT1 in Astrocytoma and its Correlation with Histopathological Grade

1. *Dr. Evana Kabir, Specialist (Pathology), United Hospital Limited, Gulshan-2, Dhaka, Bangladesh; evana19901@gmail.com
2. Professor Dr. Md Rezaul Karim Dewan, Professor, Department of Pathology, Greenlife Medical College, Ex-Professor and head, Dhaka Medical College.
3. Dr. Rejwana Nahar, Assistant Professor, Department of Pathology, Mugda Medical college, Dhaka.
4. Dr. Sadia Afroz, Medical Officer, Department of Histopathology, National Institute of Cancer Research & Hospital (NICRH), Mohakhali, Dhaka-1212, Bangladesh.
5. Dr. Shamim Rahman, Assistant Professor, Department of Pathology, Jahurul Islam Medical College, Bajitpur Bangladesh.
6. Dr. Tasmia Islam, Specialist (Pathology), Square Hospital Limited, Sher-e-Bangla Nagar, Dhaka-1205, Bangladesh.
7. Dr. Khondoker Rakibul Hoque, Senior House Officer, Department of Orthopaedics, United Hospital Limited, Gulshan, Dhaka.

*For correspondence.
Abstract
Background:  Astrocytoma comprises a group of neoplasms that differ in their location in the Central nervous system, morphologic features and invasive behavior. These are therapeutically challenging for their infiltrative growth pattern, occasionally resistant to conventional therapies and invariably high grade tumors have shown poor prognosis. Moreover biological behavior of astrocytoma and chance of recurrence cannot be ruled out by histopathological evaluation alone. So, new predictive marker for determining tumor progression is at stake.
Objectives: The purpose of the study was to evaluate WT1 expression in astrocytoma and its correlation with histological grade.
Methods:  The cross-sectional observational study was conducted in the Department of Pathology, Dhaka Medical College (DMC) from September 2019 to August 2021. Histologically diagnosed 51 cases of different grades of astrocytoma were included in this study. Immunostaining with WT1 protein was done in all cases. The data were collected and statistical analysis was done by SPSS.
Results: The mean age for grade I, II, III and IV astrocytoma’s are 14, 27.5, 38.6 and 44 years respectively. There were 30 male and 21 female patients with the ratio 1.42:1. Among 51 cases of astrocytoma’s Grade I, II ,III and IV are 10, 15, 8 and 18 in number. By Immunohistochemical study positive WT1 expression was seen in all cases of astrocytoma’s (100%). Out of 25 cases of low grade astrocytoma’s (WHO grade I and II), expression was found mild in 12 (48%) cases, moderate in 11 (54%) cases and marked only 02 (8%) cases. Regarding 26 cases of high grade astrocytoma’s (WHO grade III and IV) expression found mostly marked in 16 (61.5%) cases, moderate in 09 (34.7%) and mild only in 01 (3.8%) cases. These data shows WT1 expression increases with WHO tumor grades and significant positive (p<0.001) correlation between WT1 expression and tumor grade.
Conclusion: The study reveals positive correlation between WT1 expressions with WHO tumor grade in astrocytoma. Furthermore, evaluation of WT1 with histopathological grading may provide information about tumor progression as well as guide the clinicians for therapeutic purposes.

[Journal of Histopathology and Cytopathology, 2024 Jan; 8 (1):19-30]

DOI: https://www.doi.org/10.69950/jhc.2024.v8.i1.04
Keywords: Astrocytoma, tumor grade, WT1 expression, tumor progression, Glioblastoma

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