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jhc2025v9i1s6

Original article

Expression of Ki-67 and AgNOR in Primary Central Nervous System (CNS) Tumours

*Nahar A,1 Islam MN,2 Kabir E3

 

  1. *Dr. Asrafun Nahar, MBBS, MD(Pathology), Assistant Professor, Department of Pathology, National Institute of Laboratory Medicine and Referral Center, Sher-E-Bangla Nagar, Dhaka-1207. dr.asrafunnahar@gmail.com.
  2. Md. Nasimul Islam, MBBS, M.Phil (Pathology), Professor, Department of Pathology, Anwer Khan Modern Medical College, Dhanmondi, Dhaka.
  3. Enamul Kabir, MBBS, M. Phil, M. Sc(Pathology), Professor and Head, Department of Pathology, Popular Medical College, Dhanmondi, Dhaka

 

* For correspondence

Abstract

Background: Primary tumors in central Nervous System (CNS) include a large group of tumors. Ki-67 is usually used for determining proliferative activity, though it has some limitations. It is proved that the number or size of Argyrophilic Nucleolar Organizer Regions (AgNORs) of DNA closely correlates with cellular proliferating activities.

Objective: This study was conducted to find the correlation of expression of Ki-67 and AgNOR with different grades of primary CNS tumours.

Method: In this cross sectional study a total 85 cases with wide age range (2-70 years) were included based on selection criteria. Tissue fixation, processing, staining (hematoxylin and eosin stain, and AgNOR special stain) and immunohistochemistry were done according to the standard protocol followed by the pathology department of Sir Salimullah Medical College (SSMC) and National Institute of Neurosciences & Hospital (NINS), Dhaka.

Results: Ki-67 labeling index and mean AgNOR (mAgNOR) showed significant correlation with different grades of primary CNS tumours, as the grades increased both parameters were also increased. In intergrade comparisons, Ki-67 labeling index showed significant correlation among all the grades except between grade I & grade II and grade III & grade IV. Whereas, mean AgNOR showed significant difference among all the grades including the difference between grade I & II and grade III & IV. Positive significant correlation seen in between ki-67 Labeling Index (LI) and mean AgNOR, where the value of Spearman’s correlation coefficient was 0.514 and p value was 0.001

Conclusion: Both Ki-67 expression and mAgNOR score were increased with the increasing grade of tumours from low to high grade and positive correlation between these two parameters were also found.

[Journal of Histopathology and Cytopathology, 2025 Jan; 9 (1):38-47]

DOI: https://www.doi.org/10.69950/jhc2025v9i1s6

 

Keywords: Primary CNS tumours, mAgNOR, Ki-67 labeling index