Category: Journal

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jhc.2024.v8.i2.6


Editorial
Case Report

A Case Report on Renal Amyloidosis

*Imrana F,1 Islam SJ2

  1. *Dr. Farah Imrana, MD (Histopathology), Assistant Professor, BIHS General Hospital. farahdr02@gmail.com
  2. (Brig. Gen.) Sk. Md. Jaynul Islam, Senior Consultant, Pathology. BIRDEM Hospital. Ex. Head of the Department, Histopathology, Armed Forces Institute of Pathology (AFIP)

*For correspondence

Abstract
Amyloidosis involves the deposition of abnormal proteins in various tissue and result in progressive organ dysfunction, commonly affecting multiple organs. Two types of systemic amyloidosis are AA and AL, the former is associated with acute phase reaction and the later is composed of light chain immunoglobulin. This disease commonly affects the kidneys and is evidenced by massive proteinuria. A biopsy is the gold standard of diagnosis, with Congo Red staining revealing an apple green birefringence under polarized light microscope. We are presenting a 45 years old male who presented with rapid rising serum creatinine with history of  multiple myeloma (non secretory). There was also history of spine tuberculosis.

[Journal of Histopathology and Cytopathology, 2024 Jul; 8 (2):109-114]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i2.6

Keywords: Amyloidosis, Proteinuria
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jhc.2024.v8.i2.5


Editorial
Original Contribution
Correlation of Ki-67 Proliferating Index with Histological Types and Characterization of Mucin in Colorectal Carcinoma
*Sultana S,1 Islam N,2 Kabir E3

  1. *Dr. Sahela Sultana, MBBS. MD, Assistant Professor, Department of Pathology, Ibrahim Medical College, Dhaka. sultana.sahela83@gmail.com
  2. Dr. Nasimul Islam. MBBS. M. Phil., Professor & Head, Department of Pathology, Anwar Khan Modern Medical College & Hospital
  3. Dr. Enamul Kabir. MBBS. M.Phil. MSc(Path), Professor, Department of Pathology, Popular Medical College, Dhaka

*For Correspondence
Abstract
Background: Colorectal carcinoma is a major cause of cancer associated with a high rate of morbidity and mortality in the western world. One of the pathologic features considered to be important for prognosis is mucin production. Many authors confirmed that colon carcinomas with high mucin content tend to recur locally and carry a poor prognosis.
Aim: Correlation of Ki-67 proliferating index with different type of colorectal carcinoma as well as characterization of mucin.
Method: This cross sectional study was conducted at Sir Salimullah Medical College, Department of pathology   from July 2014 to June 2016. Ninety eight patients with colorectal carcinoma was enrolled in this  study  who underwent surgical resection of colon, adenocarcinomas. For histological classification  we used the WHO recommendation (2000) and to be more accurate we sub-classified mucinous adenocarcinomas by morphometrical  analysis in three categories: pure mucinous, with extracellular mucin more than 80% of the tumoral volume; mixed type, with 50–80%  extracellular mucin; and mixed type with less than 50% extracellular mucin and their  correlation with Ki-67 proliferating index . For histochemical investigation, we used stains such as: D- PAS and Alcian Blue. A technique of manual tissue array was employed to see Ki-67 expression by IHC method. Ki-67 is a proliferation associated nuclear antigen which can be recognized by MIB-1 monoclonal antibody.
Result: It was observed that Ki-67 labeling index was high in nonmucinous tumor  compared to mucinous adenocarcinoma and signet ring cell carcinoma which is  statistically significant (P<0.05). Histochemical stain of mucin where both D-PAS and Alcian Blue positive cases(mixed type)  are more than the Only D-PAS positive cases(pure type). Ki-67 proliferating index was also high in mixed type mucinous adenocarcinoma (<50%) compared to pure (>80%) and mixed type (50-80%). The result was statistically significant (p<0.05). Correlation of Ki-67 proliferating index with histologic type as well as mucin characterization and thereby provide information to clinician to better understanding  of the  treatment as well as prognosis.

[Journal of Histopathology and Cytopathology, 2024 Jul; 8 (2):100-108]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i2.5
Keywords: Colorectal carcinoma, Mucin, Immunohistochemistry, Ki-67.
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jhc.2024.v8.i2.4


Editorial
Original Contribution

Significance of Combined Immuno-histochemical Expression of P63 & CD56 in Papillary and Follicular Thyroid Carcinoma

 

*Majumder S,1 Khanom K,2 Ferdous JN3

  1. *Dr. Swapna Majumder, Lecturer, Department of Pathology, Rajshahi Medical College, Rajshahi, Bangladesh. dr.swapna.rmch@gmail.com
  2. Khadiza Khanom, Professor and Head, Department of Pathology, Rajshahi Medical College, Rajshahi, Bangladesh. drkhadiza68@gmail.com
  3. Jesmin Naz Ferdous, Associate Professor, Department of Pathology, Sir Salimullah Medical College, Bangladesh. limaf888@gmail.com

 

*For correspondence
Abstract
Background: Thyroid neoplasm is the commonest endocrine neoplasm. More than 80% of thyroid malignancies are papillary thyroid carcinoma (PTC) followed by follicular carcinoma (FC). The diagnosis of papillary thyroid carcinoma is based on characteristic nuclear morphology of a thyroid neoplasm. In contrast, follicular variant of PTC may cause, if the nuclear features of PTC are insufficiently appreciated, severe problems in differentiating from follicular thyroid carcinoma. Several immunohistochemical (IHC) markers such as P63 and CD56 have been recommended to differentiate between this two thyroid malignancies with overlapping histomorphology.
Objectives: Our aim was to identify the possible diagnostic role of P63 and CD56 immunoexpression that distinguish PTC, including the follicular variant from follicular thyroid carcinoma.
Methods: This cross-sectional descriptive study was conducted in the Department of Pathology, Rajshahi Medical College from March 2020 to February 2022. A total of 44 cases, histologically confirmed as papillary and follicular thyroid carcinoma were included in the study. Immunohistochemistry was done for P63 and CD56 from selected paraffin blocks.
Results: Histologically, 39 cases were diagnosed as papillary thyroid carcinoma (27 were classical PTC & 12 were FVPTC) and the rest 5 were follicular thyroid carcinoma. In this study, mean age of the patients was 40.86 ± 6.57 years (SD) and male to female ratio was 1:2.4. P63 showed 74.4% positivity with papillary thyroid carcinoma. Positive immunoreactivity of P63 was highly significant in distinguishing papillary from follicular thyroid carcinoma (P=0.003). Combined P63 positive CD56 negative (P=0.048) and P63 negative CD56 negative immunoexpression (P=0.039) were statistically significant in differentiating papillary from follicular thyroid carcinoma.
Conclusion: This study suggested that the use of P63 and CD56 may be helpful in the diagnosis of papillary and follicular thyroid carcinoma along with histopathological examination and their combination may also help in this purpose.

[Journal of Histopathology and Cytopathology, 2024 Jul; 8 (2):91-99]
DOI: https://www.doi.org/10.69950/jhc.2024.v8.i2.4

Keywords: Immunohistochemistry (IHC), PTC, FVPTC, FC, P63, CD56
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