Category: Journal

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jhc.2026.10.1.2

Journal of Histopathology and Cytopathology

Official Journal of Bangladesh Academy of Pathology

Vol 10, No 1, January 2026

Expression of Cyclin D1 in Gastric Adenocarcinoma and its Association with Staging
Lubna NT,1 Jeba R,2 Rana MS,3 Khan RR,4 Islam MM,5 Kabir E,6 *Badhan RE7

Abstract
Background: Gastric cancer is a major global health concern, ranking as the 5th most common malignancy and the 3rd leading cause of cancer-related deaths worldwide, with 783,000 deaths reported in 2018 (GLOBOCAN). Over 70% of gastric cancer cases occur in developing countries. Cyclin D1, a cell cycle regulatory protein, is implicated in various oncogenic processes, including tumor progression, invasion, and metastasis. Its overexpression has potential utility in predicting tumor behavior and guiding individualized therapy. This study aimed to assess the expression of cyclin D1 in histologically diagnosed gastric adenocarcinoma and its association with tumor stage.
Methods: A cross-sectional observational study was conducted in the Department of Pathology, Dhaka Medical College, from March 2019 to February 2022. Fifty-one cases of histologically confirmed gastric adenocarcinoma were evaluated. Routine hematoxylin and eosin staining and immunohistochemistry for cyclin D1 were performed. Clinical and demographic data were recorded, and statistical analysis was done to determine associations.
Results: The mean age of patients was 57.67±12.49 years, with a male predominance (M: F = 2.4:1). Cyclin D1 expression was positive in 33 cases (64.7%). Of these, 76.5% were in T3 stage and 93.8% in N3 stage. Cyclin D1 positivity was more frequent in advanced tumor and nodal stages.
Conclusion: Cyclin D1 expression was significantly associated with higher tumor and nodal stages in gastric adenocarcinoma. Its overexpression may serve as a prognostic biomarker and aid in risk stratification and therapeutic decision-making.

[Journal of Histopathology and Cytopathology, 2026 Jan; 10 (1):4-16]
DOI: https://www.doi.org/10.69950/jhc.2026.10.1.2
Keywords: Cyclin D1, Gastric adenocarcinoma, Immunohistochemistry, Tumor stage, Carcinoma progression

  1. Nur A Tasnim Lubna, Lecturer, Department of Pathology, Sher-E-Bangla Medical College, Barishal. tasnim.sbmc@gmail.com
  2. Ruksana Jeba, Professor and Head, Department of Pathology, Dhaka Medical College, Dhaka. Ruksanajeba67@gmail.com
  3. Md. Samsul Arafin Rana, Registrar (Hepatology), Sher-E-Bangla Medical College Hospital, Barishal. rana.mmc44@gmail.com
  4. Rizwana Rahman Khan, Assistant Professor, Department of Pathology Dhaka Medical College, Dhaka drizwanasbmc@gmail.com
  5. Mohammad Mazharul Islam, Lecturer, Department of Pathology Dhaka Medical College, Dhaka, mazharscc21@gmail.com
  6. Evana Kabir, Specialist (Histopathology), Lab Medicine, United Hospital Limited Gulsan 2, Dhaka. evanakabir1990@gmail.com
  7. *Dr. Raisa Enayet Badhan, Medical officer, Department of Microbiology, National Institute of Burn and Plastic Surgery, Dhaka. raisabadhan@gmail.com

*For correspondence

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jhc2025v9i2s1

Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2)

Editorial

Digital Pathology: Prospects and Challenges

 

*Rahman DA,1 Akter S2

 

  1. *Dr. DM Arifur Rahman, Assistant Professor, Department of Histopathology, TMSS Medical College, Bogura. arifurrahmandm@gmail.com
  2. Shormily Akter, Laboratory Scientist, TMSS Bimolecular Lab, Bogura.

*For correspondence

Abstract: Not available

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):68-69]

DOI: https://www.doi.org/10.69950/jhc2025v9i2s1

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jhc2025v9i2s3

Journal of Histopathology and Cytopathology

Official Organ of Bangladesh Academy of Pathology

Vol 9, No 2, July 2025

Conventional Polymerase Chain Reaction in the Diagnosis of Extrapulmonary Tuberculosis from Formalin-Fixed, Paraffin-Embedded Tissues

 *Adhikary L,1 Saha N,2 Tabassom T,3 Jahan D,4 Hazari TJ,5   Jeba JT,6   Rahman MH7

 *Dr Lovely Adhikary, MD (Pathology), Assistant Professor of Pathology, Ad-din Sakina Women’s Medical College, Jashore, lovelymallick71@gmail.com

  1. Dr Nirjhar Saha, MD (Pathology), Clinical pathologist, Mymensingh Medical College Hospital, Mymensingh
  2. Dr Tasnova Tabassom, MD (Pathology), Lecturer, Netrokona Medical College, Netrakona
  3. Dilshad Jahan, MD (Pathology), Assistant Professor of pathology, Barind Medical College, Rajshahi
  4. Tasnim Jahan Hazari, MD (Pathology), Clinical Pathologist, Shaheed Tajuddin Ahmed Medical College Hospital, Gazipur
  5. Jannatut Tahera Jeba, MBBS, Lecturer, Anatomy, Ad-din Sakina Women’s Medical College, Jashore
  6. Md. Hasan Hafijur Rahman, DLO, Assistant Professor (ENT), Ad-din Sakina Women’s Medical College, Jashore

     *For correspondence

Abstract
Background: Tuberculosis is one of the serious public health problems of developing countries. There is also global rise in the incidence of tuberculosis as well as in Bangladesh. The extrapulmonary tuberculosis is also common. Poverty, malnutrition, low socioeconomic condition, overcrowding and immunodeficiency are the common causes of tuberculosis in Bangladesh. This research was conducted to see the usefulness of conventional PCR in the diagnosis of extrapulmonary tuberculosis from paraffin-embedded tissues.
Methods: This was a cross-sectional observational study where molecular detection of MTB DNA by targeting the gene IS6110 of MTB with the method of conventional PCR was done from paraffin embedded tissue samples. A total of 60 cases of extrapulmonary tuberculosis that were histologically diagnosed from tissue samples on the basis of granulomatous inflammation in the proper clinical context were included in the study. Paraffin block of each case was subjected to Ziehl-Neelsen staining followed by conventional PCR examination. Results of all the cases were collected and tabulated in a data sheet. Statistical analysis was performed on the tabulated data by Fisher’s exact test.
Results: Among 60 cases 37(61.66%) cases showed negative PCR examination whereas 23(38.33%) showed positive PCR examination. Among the PCR positive 23(38.33%) cases, 22(36.6%) cases histologically contained typical granuloma. In 1(1.7%) case granuloma was not found but caseous necrosis and Langhans’ giant cells were present and PCR examination was positive. Among the PCR positive 23(38.3%) cases, 22(36.6%) cases histologically contained typical caseous necrosis but in 1(1.7%) case caseous necrosis was not found but granuloma and Langhans’ giant cells were present and PCR examination was positive. In this study among 60 cases, Z-N stain was positive in 2 cases (3.3%) and negative in 58 cases (96.7%). Among the Z-N stain positive 2(3.3%) cases, PCR was positive in one case and in other case PCR examination was negative. Statistically significant association was not found with PCR examination results with granuloma (P-0.343) and caseous necrosis (p-0.675).
Conclusion: The results of the present study suggested that PCR can be considered as a diagnostic modality in the challenging cases of extrapulmonary tuberculosis from FFPE tissue samples by demonstrating the presence of MTB specific DNA.

[Journal of Histopathology and Cytopathology, 2025 Jul; 9 (2):78-88]
DOI: https://www.doi.org/10.69950/jhc2025v9i2s3

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