Category: Journal

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jhc2026v10i2s13


Case Report
BRAF V600E-Positive Classical Papillary Thyroid Carcinoma: Integrating Histopathological Diagnosis with Next-Generation Sequencing for Personalized Oncological Management – A Case Report
*Rahman DA1, Akter S2

  1. *Dr. DM Arifur Rahman, Associate Professor, Department of Histopathology, TMSS Medical College, Bogura. arifurrahmandm@gmail.com. ORCID: https://orcid.org/ 0009-0007-2493-9230
  2. Dr. Shormily Akter, Scientist, TMSS Biomolecular Lab, Bogura. ORCID: https://orcid.org/0009-0007-2493-9230

*For correspondence
Abstract
Background: Papillary thyroid carcinoma (PTC) is the most prevalent thyroid malignancy globally, with BRAF V600E being its most common somatic driver mutation. This mutation constitutively activates the MAPK/ERK signalling pathway, contributing to aggressive clinicopathological behaviour and radioiodine (RAI) refractoriness.
Case Presentation: We report a 40-year-old female who presented with a thyroid nodule and was histopathologically diagnosed with classical PTC. Formalin-fixed paraffin-embedded (FFPE) tissue was subjected to targeted next-generation sequencing (NGS) using the oncoReveal™ Solid Tumor v2 Panel (48 genes) on the Illumina NextSeq™ 1000 platform. NGS revealed a pathogenic somatic BRAF c.1799T>A (p.Val600Glu) single nucleotide variant (SNV) in exon 15.
Conclusion: This case illustrates the complementary roles of histopathology and NGS in the WHO 2022 molecular classification framework for PTC. The identification of a pathogenic BRAF V600E mutation confirms molecular diagnosis, supports RAI-refractoriness risk assessment, and enables eligibility for Dabrafenib/Trametinib combination therapy, exemplifying precision oncology in thyroid cancer.
[Journal of Histopathology and Cytopathology, 2026 Jul; 10 (2):170-178]
DOI: https://www.doi.org/10.69950/jhc.2026.10.2.13

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jhc2026v10i2


Editorial
1. ASCO 2026 Guideline: Rethinking PD-L1 Testing
Rahman DA
Original Contributions
2.Lupus Nephritis; an Unrevealed Morbidity in Bangladeshi Population- A Clinical and Histomorphological Study
Imrana, Islam SJ, Rahman TS, Azad A
3.Stromal Expression of CD10 in Oral Squamous Cell Carcinoma and its Association with Histological Grade
Hasnain M, Jeba R, Doly NJ, Hasan M, Nahar R, Nupur FP
4.Spectrum of Hepatic Lesions Diagnosed by Image-Guided Cytology and Core Needle Biopsy: A Retrospective Clinicopathological Study
Quruni MO, Hossain MS, Afrin SS, Saha MK, Alam SN, Nupur FP, Jahan PA
5.Immunohistochemical Expression of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN/CD147) in Renal Cell Carcinoma and Its Association with Histological Grade and Pathological Stage
Mahmud S, Bhuiyan AZ,Hossain SA, Yasmin R,Saha MK,Masud MR,Shakila J, Afrin S
6.Histomorphological Pattern of Sellar and Suprasellar Region Tumor in Tertiary Level Hospital of Bangladesh
Shirin S, Jeba R, Wahid SR, Nupur FP, Jannat MR
7.Vimentin Expression in Renal Cell Carcinoma and It’s Association with Histological Grade and Pathological Stage
Adhikary P, Begum S, Ferdous JN, Saha AK, Papry A, Emita U
8.Vimentin Expression in Infiltrating Ductal Carcinoma NOS of Breast and its Association with Clinicopathological Characteristics
Tahsin KN, Doly NJ, Rashid HO,Yeamin MA, Sultana S,Khan RR, Jeba R
9.Immunohistochemical Evaluation of PD-L1 Expression in Triple-Negative Breast Cancer: An Analysis of 83 Cases
Alam MM, Dey BP, Akhtaruzzaman M, Jahan I, Nahid MS, Rahman MZ
10.PD-L1 Immunoexpression in Selected Cases of Lung Carcinoma: A Retrospective Cross-Sectional Study from a Tertiary Care Center in Bangladesh
Khanam KF, Ayeeda AS, Omar T, Bhuiyan MT, Khanam KA, Bhadra S,Manik MM
Case Reports
11.Heterotopic Ossification of the Gallbladder Associated with Chronic Cholecystitis: A Case Report
Anjum R, Hossain GZ
12.Pulmonary Sclerosing Pneumocytoma: Diagnostic Challenges in Frozen Section – A Case Report and Literature Review
Anjum R, Hossain Bhuiyan MZ
13.BRAF V600E-Positive Classical Papillary Thyroid Carcinoma: Integrating Histopathological Diagnosis with Next-Generation Sequencing for Personalized Oncological Management – A Case Report
Rahman DA, Akter S
14. Eosinophilic Mastitis: A Rare Case Report
Hussain AR, *Khanam KF, Akhter S, Omar T, Ayeeda AS,  Khanam KA

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jhc.2026.10.1.9

Journal of Histopathology and Cytopathology
Vol 10, Issue 1, 2026

Comparative Analysis of Serum β-hCG Levels in Complete and Partial Hydatidiform Moles
 Rahman MM,1 Kabir AN,2 Islam T,3 Shiraj-Um-Mahmuda S,4 Karim R,5 *Shabnam US6

Abstract
Introduction: Hydatidiform mole (HM) is a gestational trophoblastic disorder caused by abnormal fertilization, resulting in a non-viable pregnancy with trophoblastic hyperplasia. Differentiation between complete hydatidiform mole (CHM) and partial hydatidiform mole (PHM) is clinically important because CHM carries a higher risk of persistent trophoblastic disease. Serum β-hCG is often markedly elevated in molar pregnancy and may assist in distinguishing CHM from PHM. This study aimed to evaluate pretreatment β-hCG levels among different types of hydatidiform mole and assess their diagnostic association.
Methods: This cross-sectional study included 57 histopathologically diagnosed cases of HM collected from the BMU and private laboratories in Dhaka. Pretreatment serum β-hCG levels were obtained for all patients. Based on histopathological criteria, cases were grouped into CHM, PHM, and indeterminate categories. Statistical analysis using Mann-Whitney and Kruskal-Wallis tests was performed to compare β-hCG levels between groups.
Results: Of the 57 cases, 36 were CHM, 13 were PHM, and 8 were categorized as indeterminate. Serum β-hCG levels showed statistically significant variation among the three groups, with CHM showing markedly higher levels than PHM. Elevated β-hCG demonstrated a strong association with the histopathological diagnosis of HM.
Conclusion: Pretreatment serum β-hCG level is a valuable adjunct in differentiating complete from partial hydatidiform mole. When used alongside histopathological assessment, β-hCG can enhance diagnostic confidence and guide appropriate clinical management.

[Journal of Histopathology and Cytopathology, 2026 Jan; 10 (1):71-83]
DOI: https://www.doi.org/10.69950/jhc.2026.10.1.9

Keywords: β-hCG, complete hydatidiform mole, partial hydatidiform mole, trophoblastic disease.

  1. Dr. Mohammad Mosiur Rahman, Associate Professor, Patholog), Bangladesh Medical University, Bangladesh. mosiurpath@bsmmu.edu.bd.
  2. Dr. AKM Nurul Kabir, Professor, Pathology, Bangladesh Medical University, Dhaka
  3. Dr. Tasmia Islam, Assistant Professor, Pathology, Bangladesh Medical University. Dhaka
  4. Dr. Syeeda Shiraj-Um-Mahmuda, MD (Pathology), OSD, Directorate General of Health Services (DGHS).
  5. Dr. Rezwana Karim, Associate Professor (C.C), Pathology, US – Bangla Medical College and Hospital, Dhaka
  6. *Dr. Ummey Salma Shabnam, Assistant Professor (Histopathology), National Institute of Cancer Research and Hospital, Place. salmamithun@gmail.com. Orcid id: 0009-0005-1751-116X,

*For correspondence

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